Cancer-associated fibroblasts affect breast cancer cell gene expression, invasion and angiogenesis

Cellular Oncology (Dordrecht)
Noemi EiroFrancisco J Vizoso

Abstract

It has been reported that stromal cell features may affect the clinical outcome of breast cancer patients. Cancer associated fibroblasts (CAFs) represent one of the most abundant cell types within the breast cancer stroma. Here, we aimed to explore the influence of CAFs on breast cancer gene expression, as well as on invasion and angiogenesis. qRT-PCR was used to evaluate the expression of several cancer progression related genes (S100A4, TGFβ, FGF2, FGF7, PDGFA, PDGFB, VEGFA, IL-6, IL-8, uPA, MMP2, MMP9, MMP11 and TIMP1) in the human breast cancer-derived cell lines MCF-7 and MDA-MB-231, before and after co-culture with CAFs. Stromal mononuclear inflammatory cell (MIC) MMP11 expression was used to stratify primary tumors. In addition, we assessed the in vitro effects of CAFs on both MDA-MB-231 breast cancer cell invasion and endothelial cell (HUVEC) tube formation. We found that the expression levels of most of the genes tested were significantly increased in both breast cancer-derived cell lines after co-culture with CAFs from either MMP11+ or MMP11- MIC tumors. IL-6 and IL-8 showed an increased expression in both cancer-derived cell lines after co-culture with CAFs from MMP11+ MIC tumors. We also found that the invasive and ...Continue Reading

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Citations

Sep 16, 2018·Analytical and Bioanalytical Chemistry·Raquel González de VegaAlfredo Sanz-Medel
Jul 26, 2019·Histopathology·Noemi EiroFrancisco J Vizoso
Dec 31, 2019·The Journal of International Medical Research·Xin-Da SongBin Liu
Jan 5, 2020·Cellular Oncology (Dordrecht)·Daniel CruceriuIoana Berindan-Neagoe
Mar 3, 2020·Integrative Biology : Quantitative Biosciences From Nano to Macro·Molly M MorganDavid J Beebe
Apr 7, 2020·Expert Opinion on Therapeutic Targets·Rosamaria LappanoErnestina Marianna De Francesco
Jun 9, 2019·Cellular Oncology (Dordrecht)·Sukru GulluogluOmer Faruk Bayrak
Jun 5, 2020·Cellular Oncology (Dordrecht)·Fei HuangQianming Chen
Jun 6, 2020·Frontiers in Immunology·Alireza Labani-MotlaghAngelica Loskog
May 31, 2019·Cellular Oncology (Dordrecht)·Arash SalmaninejadAmirhossein Sahebkar
Apr 14, 2019·Cellular Oncology (Dordrecht)·Afsane BahramiAmirhossein Sahebkar
Nov 5, 2019·Cellular Oncology (Dordrecht)·Kim Jun ChengZaridatul Aini Ibrahim
Oct 4, 2019·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Ana S DiasIola F Duarte
Jan 6, 2021·International Journal of Molecular Sciences·Noemi EiroFrancisco J Vizoso
Jan 6, 2021·Journal of Mammary Gland Biology and Neoplasia·María Belén GiorelloNorma Alejandra Chasseing
Jan 10, 2021·International Journal of Molecular Sciences·Griselda A Cabral-PachecoMargarita L Martinez-Fierro
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Apr 6, 2021·World Journal of Biological Chemistry·Rama Rao MallaGanji Purnachandra Nagaraju
May 18, 2020·Cellular Oncology (Dordrecht)·Meysam YousefiAlireza Pasdar
Aug 3, 2021·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Shidong TanZhongqun Wang
Jul 17, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Priyanila MageshPadmanaban S Suresh
Aug 5, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Bing MaHai-Hong Zhang
Oct 7, 2021·Journal of Cell Communication and Signaling·Zihong WuMasoud Najafi

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Methods Mentioned

BETA
flow cytometry

Software Mentioned

LI
COR Odyssey
AngioTool

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