Cancer cell sensitivity to arginine deprivation in vitro is not determined by endogenous levels of arginine metabolic enzymes

Cell Biology International
Yaroslav P BobakOleh V Stasyk

Abstract

Single amino acid Arg (arginine) deprivation is currently considered as a therapeutic approach to treat certain types of tumours; the molecular mechanisms that underlie tumour cell sensitivity or resistance to Arg restriction are still little understood. Here, we address the question of whether endogenous levels of key Arg metabolic enzymes [catabolic: arginases, ARG1 (arginase type 1) and ARG2 (arginase type 2), and anabolic: OTC (ornithine transcarbamylase) and ASS (argininosuccinate synthetase)] affect cellular responses to arginine deprivation in vitro. Human epithelial cancer cells of different organs of origin exhibiting variable sensitivity to Arg deprivation provided the experimental models. Neither the basal expression status of the analysed enzymes, nor their changes upon arginine withdrawal correlated with cancer cell sensitivity to arginine deprivation. However, the ability to utilize exogenous Arg precursors (ornithine and citrulline) for growth in Arg-deficient medium strongly correlated with expression of the corresponding enzymes, OTC and ASS. We also observed that OTC expression was below the level of detection in all the types of tumour cells analysed, suggesting that in vitro, at least for them, Arg is an ess...Continue Reading

References

Apr 24, 2003·European Journal of Biochemistry·Annie HussonAlain Lavoinne
May 15, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Francesco IzzoSteven A Curley
Aug 23, 2005·Cancer Letters·Denys N WheatleyElaine Campbell
Oct 20, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Paolo A AsciertoTheodore F Logan
Feb 14, 2006·The American Journal of Clinical Nutrition·Sidney M Morris
Mar 31, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Evan S GlazerFrancesco Izzo

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Citations

Jan 12, 2016·Experimental Cell Research·Bozhena O Vynnytska-MyronovskaOleh V Stasyk
Nov 8, 2015·The International Journal of Biochemistry & Cell Biology·Yaroslav BobakOleh Stasyk
Jun 8, 2011·International Journal of Cancer. Journal International Du Cancer·Bozhena Vynnytska-MyronovskaLeoni A Kunz-Schughart
Sep 19, 2014·Cell Biology International·Oleh V StasykAndriy A Sibirny
Jul 6, 2014·BioMed Research International·Galyna ShuvayevaCiro Isidoro
Nov 5, 2016·Expert Opinion on Therapeutic Patents·H S FernandesN M F S A Cerqueira
Feb 19, 2020·Cell Biology International·Galyna Y ShuvayevaOleh V Stasyk
Mar 22, 2020·Pharmaceutics·Larisa KolevaElena Sinauridze
Nov 24, 2017·International Journal of Cancer. Journal International Du Cancer·Carmela De SantoFrancis Mussai
Jan 30, 2021·Frontiers in Pharmacology·Ziyu WangDianwen Ju
Jun 17, 2021·Scientific Reports·Rósula García-NavasFaustino Mollinedo

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