Cancer cell targeting and therapeutic delivery of silver nanoparticles by mesoporous silica nanocarriers: insights into the action mechanisms using quantitative proteomics.

Nanoscale
Sandra Montalvo-QuirosJose L Luque-Garcia

Abstract

An approach for safely delivering AgNPs to cancer cells and the evaluation of the affected cellular mechanism are presented. The use of mesoporous silica nanoparticles (MSNs) as nanovehicles decorated with transferrin (Tf, targeting agent) provides a nanoplatform for the nucleation and immobilization of AgNPs (MSNs-Tf-AgNPs). We performed the physico-chemical characterization of the nanosystems and evaluated their therapeutic potential using bioanalytical strategies to estimate the efficiency of the targeting, the degree of cellular internalization in two cell lines with different TfR expression, and the cytotoxic effects of the delivered AgNPs. In addition, cellular localization of the nanosystems in cells has been evaluated by a transmission electron microscopy analysis of ultrathin sections of human hepatocarcinoma (HepG2) cells exposed to MSNs-Tf-AgNPs. The in vitro assays demonstrate that only the nanosystem functionalized with Tf is able to transport the AgNPs inside the cells which overexpress transferrin receptors. Therefore, this novel nanosystem is able to deliver AgNPs specifically to cancer cells overexpressing Tf receptors and offers the possibility of a targeted therapy using reduced doses of silver nanoparticles ...Continue Reading

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Citations

Mar 23, 2019·Expert Opinion on Drug Delivery·Rafael R CastilloMaría Vallet-Regí
May 14, 2020·Nanomaterials·Miguel Gisbert-Garzarán, María Vallet-Regí
Oct 4, 2020·IET Nanobiotechnology·Hasnat Tariq, Syed Ali Imran Bokhari
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Dec 20, 2020·Pharmaceutics·Sandra Montalvo-QuirósJose L Luque-Garcia
Dec 24, 2020·International Journal of Molecular Sciences·Miguel Gisbert-GarzaránMaría Vallet-Regí
Nov 2, 2020·Colloids and Surfaces. B, Biointerfaces·Sandra Montalvo-QuirósJose L Luque-Garcia
May 6, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Fatih KurtulduAldo R Boccaccini
Dec 26, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Paulina SokołowskaBogdan Walkowiak
Jan 13, 2022·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Xuan HeChao Yang

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Datasets Mentioned

BETA
CAA76847.1
AAB22049.1

Methods Mentioned

BETA
NMR
dynamic
scanning
transmission electron microscopy
flow cytometry
Assay
dynamic light scattering
X-ray

Software Mentioned

SILAC
QuiXoT
ZoomScan
MASCOT
Eksigent
Expasy

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