Cancer cells grown in 3D under fluid flow exhibit an aggressive phenotype and reduced responsiveness to the anti-cancer treatment doxorubicin.

Scientific Reports
Tayebeh AzimiMiriam V Dwek

Abstract

3D laboratory models of cancer are designed to recapitulate the biochemical and biophysical characteristics of the tumour microenvironment and aim to enable studies of cancer, and new therapeutic modalities, in a physiologically-relevant manner. We have developed an in vitro 3D model comprising a central high-density mass of breast cancer cells surrounded by collagen type-1 and we incorporated fluid flow and pressure. We noted significant changes in cancer cell behaviour using this system. MDA-MB231 and SKBR3 breast cancer cells grown in 3D downregulated the proliferative marker Ki67 (P < 0.05) and exhibited decreased response to the chemotherapeutic agent doxorubicin (DOX) (P < 0.01). Mesenchymal markers snail and MMP14 were upregulated in cancer cells maintained in 3D (P < 0.001), cadherin-11 was downregulated (P < 0.001) and HER2 increased (P < 0.05). Cells maintained in 3D under fluid flow exhibited a further reduction in response to DOX (P < 0.05); HER2 and Ki67 levels were also attenuated. Fluid flow and pressure was associated with reduced cell viability and decreased expression levels of vimentin. In summary, aggressive cancer cell behaviour and reduced drug responsiveness was observed when breast cancer cells were main...Continue Reading

References

Jan 1, 1997·British Journal of Cancer·Y BoucherR K Jain
May 2, 2001·The Journal of Biological Chemistry·S M EllerbroekM S Stack
Jul 25, 2006·Nature Reviews. Cancer·Andrew I Minchinton, Ian F Tannock
Oct 20, 2006·IUBMB Life·Yoshifumi Itoh
Dec 16, 2006·Journal of Cellular Biochemistry·Dai Fukumura, Rakesh K Jain
Jan 28, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Einar K RofstadKanthi Galappathi
Sep 9, 2010·Current Opinion in Cell Biology·Mikala EgebladValerie M Weaver
Apr 7, 2011·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Nadine Rohwer, Thorsten Cramer
Apr 19, 2011·Journal of Cell Communication and Signaling·Agata NygaMarilena Loizidou
Jan 13, 2012·Molecular Biology of the Cell·Jennifer L LeightChristopher S Chen
Feb 20, 2013·Journal of Cell Science·Frances R BalkwillThorsten Hagemann
Apr 30, 2013·Acta Biomaterialia·Agata NygaUmber Cheema
Sep 18, 2013·International Journal of Molecular Medicine·Tao YuLongjiang Li
Oct 3, 2013·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·T YuL Li
Aug 30, 2014·Cancer Management and Research·Jennifer M Munson, Adrian C Shieh
Feb 9, 2016·Integrative Biology : Quantitative Biosciences From Nano to Macro·Alexandra S Piotrowski-DaspitCeleste M Nelson
Mar 2, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A CaseyH J Byrne
Dec 7, 2016·OncoTargets and Therapy·NamHuk BaekSeong Soo A An
Jan 18, 2017·Nature Communications·Hyun Jung LeePamela L Wenzel
Jan 20, 2017·Oncology Letters·Kamil PohlodekDaphne Gschwantler-Kaulich
Feb 12, 2017·Scientific Reports·Shawn P CareyCynthia A Reinhart-King
Mar 10, 2017·Scientific Reports·Tarig MagdeldinUmber Cheema
Jul 4, 2017·Future Science OA·John Greenman
Feb 18, 2018·Scientific Reports·Shantanu PradhanElizabeth A Lipke
May 22, 2018·Frontiers in Oncology·Vasiliki Gkretsi, Triantafyllos Stylianopoulos
Jun 9, 2018·Frontiers in Bioengineering and Biotechnology·Jorge A BelgodereJangwook P Jung
May 11, 2019·PloS One·Michael E BregenzerGeeta Mehta

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Citations

Feb 6, 2021·Annals of Biomedical Engineering·Alice PasiniEmanuele Giordano
Apr 30, 2021·Frontiers in Bioengineering and Biotechnology·Judith PapeUmber Cheema

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Methods Mentioned

BETA
confocal microscopy
fluorescence microscopy
chip
electrophoresis

Software Mentioned

Vivo
OriginPro
OriginLab
Quasi

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