Cancer-Cells on Chip for Label-Free Detection of Secreted Molecules

Biosensors
Ophélie I BerthuyChristophe A Marquette

Abstract

In the present report, we are making the proof of concept of cell small populations (from 1 to 100 cells) spotting, culture and secretion detection on a gold surface. In order to keep the cells in a hydrated environment during the robotized micropipetting and to address different cell lines on a single chip, a biocompatible alginate polymer was used. This approach enables the encapsulation of the cell in a very small volume (30 nL), directly on the substrate and permits a precise control of the number of cells in each alginate bead. After 24 h of culture, the adherent cells are ready for surface plasmon resonance imaging (SPRi) experimentation. To enable the detection of secreted proteins, various antibodies are immobilized in an organized manner on a SPRi sensor and permitted the multiplex detection of different proteins secreted by the different cultured cell lines. Evidence of the real-time detection will be presented for Prostate Specific Antigen (PSA) and β-2-microglobulin (B2M) secreted by prostate cancer cells following induction by dihydrotestosterone (DHT). Different kinetics for the two secreted proteins were then demonstrated and precisely determined using the chip.

References

Mar 17, 2004·Biomaterials·E A RothT Boland
Feb 7, 2006·Biomaterials·Didier FalconnetMarcus Textor
Jul 29, 2008·Analytical Chemistry·Tiago G FernandesJonathan S Dordick
Dec 31, 2010·Critical Reviews in Biotechnology·Hadar Ben-YoavShimshon Belkin
Jun 10, 2011·Lab on a Chip·Azmi YusofPeter Koltay
Feb 22, 2012·Clinics in Laboratory Medicine·Panga Jaipal ReddySanjeeva Srivastava
Sep 8, 2012·Chemical Communications : Chem Comm·Chang LiuChen-Zhong Li

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Citations

Mar 2, 2019·Annual Review of Analytical Chemistry·Markéta BockováJiří Homola
Apr 14, 2018·Analytical and Bioanalytical Chemistry·Yu-Wen Su, Wei Wang
Aug 21, 2020·Journal of Agricultural and Food Chemistry·Bin Wang, Bosoon Park
Sep 7, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Meriem ChadliAgnès Girard-Egrot

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Methods Mentioned

BETA
biosensing
biosensors
biosensor
surface plasmon resonance
chip
ELISA

Software Mentioned

Genoptix
Ascent

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