Cancer-Specific MicroRNA Analysis with a Nonenzymatic Nucleic Acid Circuit

ACS Applied Materials & Interfaces
Dan ZhuJie Chao

Abstract

Sensitive detection of gastric cancer-related biomarkers in human serum provides a promising means for early cancer diagnosis. Herein, we report the design of a nucleic acid circuit for gastric cancer-related microRNA-27a (miRNA-27a) detection based on dual toehold-mediated circular strand displacement amplification (CSDA). In the presence of miRNA-27a, the hybridization between miRNA-27a and probe DNA on magnetic beads through toehold 1 leads to the release of fluorescent DNA and the exposure of a new toehold 2 on linker DNA. After hybridization with catalytic DNA, CSDA is initiated and target miRNA-27a is released to participate in the next cyclic reaction; therefore, a greatly enhanced fluorescence signal is produced. The efficient magnetic separation makes the sensitive detection of miRNA-27a be accomplished within 45 min. With the efficient CSDA, the detection limit of the system (0.8 pM) is ∼100 folds lower than that of the system based on strand displacement without CSDA (79.3 pM). Furthermore, the system also showed good stability and sensitivity to discriminate single-base mismatch, which allows the detection of miRNA-27a in human serum samples. This study provides a novel platform and approach for the rapid quantitati...Continue Reading

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Citations

Feb 23, 2020·Beilstein Journal of Nanotechnology·María Sanromán Iglesias, Marek Grzelczak
Jun 24, 2020·Angewandte Chemie·Linlin YangDa Han
Jan 3, 2020·Topics in Current Chemistry·Weiyang TangYizhen Liu
Sep 11, 2021·Analytical Chemistry·Dan ZhuLianhui Wang

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