Cancer spheroids derived exosomes reveal more molecular features relevant to progressed cancer.

Biochemistry and Biophysics Reports
Junfang TuMei He

Abstract

Cancer cell spheroids have been shown to be more physiologically relevant to native tumor tissue than monolayer 2D culture cells. Due to enhanced intercellular communications among cells in spheroids, spheroid secreted exosomes which account for transcellular transportation should exceed those from 2D cell culture and may display a different expression pattern of miRNA or protein. To test this, we employed a widely used pancreatic cancer cell line, PANC-1, to create 3D spheroids and compared exosomes generated by both 2D cell culture and 3D PANC-1 spheroids. We further measured and compared exosomal miRNA and GPC-1 protein expression with qRT-PCR and enzyme-linked immunosorbent assay, respectively. It showed that PANC-1 cells cultured in 3D spheroids can produce significantly more exosomes than PANC-1 2D cells and exosomal miRNA and GPC-1 expression derived from spheroids show more features relevant to the progression of pancreatic cancer. These findings point to the potential importance of using spheroids as in vitro model to study cancer development and progression.

References

May 15, 1975·International Journal of Cancer. Journal International Du Cancer·M LieberG Todaro
Feb 24, 2006·Nature Reviews. Molecular Cell Biology·Linda G Griffith, Melody A Swartz
Mar 25, 2006·Nature Reviews. Cancer·Aurora Esquela-Kerscher, Frank J Slack
Apr 27, 2007·Nature Genetics·Scott M Hammond
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Jun 9, 2009·International Journal of Surgery·Oliver StrobelJens Werner
Jun 3, 2010·Cold Spring Harbor Protocols·Donald C RioTimothy W Nilsen
Jan 29, 2013·Journal of Cellular Biochemistry·Xiu-Li JinYu-Yang Jiang
Feb 13, 2013·Proteomics·Dong-Sic ChoiYong Song Gho
Mar 25, 2014·Current Opinion in Genetics & Development·Norman Sachs, Hans Clevers
Apr 17, 2014·International Journal of Molecular Sciences·Chunlong LiYunfu Cui
May 17, 2014·Assay and Drug Development Technologies·Rasheena EdmondsonLiju Yang
Nov 25, 2014·Biophysical Journal·Morgan DelarueGiovanni Cappello
Apr 30, 2015·Methods : a Companion to Methods in Enzymology·Steven McClellanLaurie Owen
Feb 26, 2016·Nature Cell Biology·Aliya FatehullahNick Barker
Apr 2, 2016·The Journal of Clinical Investigation·Raghu Kalluri
Jun 10, 2016·Theranostics·Aranzazu VillasanteGordana Vunjak-Novakovic
Nov 8, 2017·Biochemical and Biophysical Research Communications·Atsushi MasamuneTooru Shimosegawa
Dec 19, 2017·Biochemical and Biophysical Research Communications·Lilite SadovskaAija Linē
Apr 26, 2018·Nature Reviews. Cancer·Jarno Drost, Hans Clevers
Jun 28, 2018·World Journal of Urology·Cassandra Ringuette-GouletFrédéric Pouliot
Dec 7, 2018·Biochemical and Biophysical Research Communications·Egor E DiakonovAnna S Tsimokha
Feb 27, 2019·RNA Biology·Yi-Fan XuWei-Qun Ding
Apr 6, 2019·Cell·Dennis K JeppesenRobert J Coffey
Apr 10, 2019·Nature Protocols·Koh-Ei ToyoshimaTakashi Tsuji
May 3, 2019·Biochimica Et Biophysica Acta. Reviews on Cancer·Lin Zhang, Dihua Yu
Sep 12, 2019·Scientific Reports·Sirisha ThippabhotlaMei He
May 8, 2020·BMC Gastroenterology·Caterina VicentiniAldo Scarpa

❮ Previous
Next ❯

Software Mentioned

SPSS16

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer
T Nederman, P Twentyman
© 2021 Meta ULC. All rights reserved