Cancer vaccines

Current Protocols in Human Genetics
T F Greten, E M Jaffee

Abstract

This unit describes the use of retroviral vectors that can be successfully employed for gene transfer into both primary tumor cultures and established cell lines. The unit includes procedures for assaying the stability of the vaccine following gene transfer. Techniques for maintaining the retroviral producer lines and titering the retroviral vectors are also described. A protocol for frozen storage of the vaccine (transduced tumor cells) is provided. In addition, methods are described for characterizing the vaccine cells following gene transfer. Directions for testing the expression of the transferred gene in the transfected tumor line are also given. The final protocol provides suggestions for designing in vivo animal experiments and discusses what has to be observed in a clinical setting.

References

May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J M WilsonR C Mulligan
Jan 1, 1995·Annual Review of Immunology·D M Pardoll
Jan 1, 1994·Annual Review of Immunology·T BoonA Van Pel
Jun 1, 1993·Immunology Today·D M Pardoll
May 1, 1996·Journal of Immunotherapy with Emphasis on Tumor Immunology : Official Journal of the Society for Biological Therapy·E M JaffeeD M Pardoll

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Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.