Cancerous inhibitor of protein phosphatase 2A promotes premature chromosome segregation and aneuploidy in prostate cancer cells through association with shugoshin

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Rajash PallaiLyndi M Rice

Abstract

Yeast two-hybrid (Y2H) studies have shown that cancerous Inhibitor of protein phosphatase 2A (CIP2A) interacted with several proteins, including leucine-rich repeat-containing protein 59 (LRRC59), suggesting that CIP2A may interact with the chromosome maintenance protein, shugoshin (Sgol1). We previously showed that LRRC59 interacted with CIP2A, which was required for CIP2A nuclear localization. Thus, we predicted that CIP2A and Sgol1 may also interact. Sgol1 is a nuclear protein that regulates chromosome segregation during cell division via protection of cohesin ring proteins. Here, we demonstrated that Sgol1 and the C-terminus of CIP2A interact in prostate carcinoma cell lines in a protein phosphatase 2A (PP2A)-dependent manner. Moreover, we demonstrated that depletion of CIP2A in PC-3 cells decreases premature chromosome segregation, whereas overexpression of CIP2A in an immortalized prostate cell line increases premature chromosome segregation. Importantly, we further showed that CIP2A depletion decreases the incidence of aneuploidy and stabilizes cohesin complex proteins, while overexpression of CIP2A destabilizes Sgol1. Thus, our findings strongly suggest that CIP2A promotes cell cycle progression, premature chromosome se...Continue Reading

References

Apr 28, 2001·Neoplasia : an International Journal for Oncology Research·B BeheshtiJ A Squire
Mar 17, 2006·Nature·Tomoya S KitajimaYoshinori Watanabe
Nov 7, 2006·Developmental Cell·Jun DaiJonathan M G Higgins
Nov 23, 2006·Cell·Stephanie KuengJan-Michael Peters
Jul 17, 2007·Cell·Marc Mumby
Jul 17, 2007·Cell·Melissa R JunttilaJukka Westermarck
Nov 16, 2007·Journal of Cell Science·Masato NakajimaToru Hirota
Mar 11, 2008·Trends in Molecular Medicine·Jukka Westermarck, William C Hahn
Jul 1, 2008·Biochimica Et Biophysica Acta·Pieter J A EichhornRené Bernards
Mar 21, 2009·Genes & Development·Dean CliftAdele L Marston
Sep 1, 2009·Molecular Cell·Zheng XuWenqing Xu
Sep 18, 2009·Developmental Cell·Anna V Kateneva, Jonathan M G Higgins
Oct 23, 2010·Journal of Experimental & Clinical Cancer Research : CR·Markku H VaaralaAri Ristimäki
Sep 8, 2011·British Journal of Cancer·C BöckelmanA Ristimäki
Feb 14, 2012·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Hao-Wei TengKuen-Feng Chen
Feb 19, 2013·Seminars in Cell & Developmental Biology·Lauren M ZasadilBeth A Weaver
Apr 10, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Lei WangHong-Yong Cao
Aug 1, 2013·Proceedings of the National Academy of Sciences of the United States of America·Tomoko NishiyamaJan-Michael Peters
Nov 8, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jinming HuangChun Yang

❮ Previous
Next ❯

Citations

Apr 3, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Rajash PallaiLyndi M Rice
Aug 4, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Paula González-AlonsoFederico Rojo
Apr 4, 2017·Scientific Reports·Beatriz Navarro-DomínguezJuan Pedro M Camacho
Feb 9, 2017·EMBO Reports·Jiao WangJukka Westermarck

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.