Candida albicans cyclin Clb4 carries S-phase cyclin activity.

Eukaryotic Cell
Ayala Ofir, Daniel Kornitzer

Abstract

Cyclin-dependent kinases (CDKs) are key regulators of eukaryotic cell cycle progression. The cyclin subunit activates the CDK and also imparts to the complex, at least in some cases, substrate specificity. Saccharomyces cerevisiae, an organism in which the roles of individual cyclins are best studied, contains nine cyclins (three G(1) cyclins and six B-type cyclins) capable of activating the main cell cycle CDK, Cdc28. Analysis of the genome of the pathogenic yeast Candida albicans revealed only two sequences corresponding to B-type cyclins, C. albicans Clb2 (CaClb2) and CaClb4. Notably, no homolog of the S. cerevisiae S-phase-specific cyclins, Clb5/Clb6, could be detected. Here, we performed an in vitro analysis of the activity of CaClb2 and CaClb4 and of three G(1) cyclins, as well as an analysis of the phenotype of S. cerevisiae cells expressing CaClb2 or CaClb4 instead of Clb5. Remarkably, replacement of CLB5 by CaCLB4 caused rapid diploidization of S. cerevisiae. In addition, both in vivo and in vitro analyses indicate that, in spite of the higher sequence similarity of CaClb2 to Clb5/Clb6, CaClb4 is the functional homolog of Clb5/Clb6. The activity of a CaClb2/CaClb4 cyclin hybrid suggests that the cyclin box domain of Ca...Continue Reading

References

Jul 1, 1992·Molecular Biology of the Cell·I FitchB Futcher
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·J A KahanaP A Silver
Jul 25, 1997·Science·A F StraightA W Murray
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·B A SchulmanE Harlow
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·M D Mendenhall, A E Hodge
May 18, 1999·Molecular and Cellular Biology·J D LoebH Liu
Aug 13, 1999·Molecular Cell·F R CrossM D Jacobson
Feb 26, 2000·Molecular Biology of the Cell·C Martín-CastellanosS Moreno
Jun 10, 2000·Molecular and Cellular Biology·F R Cross, M D Jacobson
Jun 21, 2002·Methods in Enzymology·Daniel Kornitzer
Dec 22, 2004·Cell Cycle·Vincent ArchambaultFrederick R Cross
Jan 12, 2005·Eukaryotic Cell·Bernardo Chapa y LazoPeter Sudbery
Jan 12, 2005·Eukaryotic Cell·Catherine Bachewich, Malcolm Whiteway
Jan 22, 2005·Nucleic Acids Research·Kazutaka KatohTakashi Miyata
Feb 11, 2005·Eukaryotic Cell·Tsvia GildorDaniel Kornitzer
Apr 9, 2005·Molecular Biology of the Cell·Avigail Atir-LandeDaniel Kornitzer
May 13, 2005·Molecular Biology of the Cell·Eric S BensenJudith Berman
Jun 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Fangfang Hu, Oscar M Aparicio
May 19, 2006·The Journal of Biological Chemistry·Kin-Yip ChengLouise N Johnson
Jun 24, 2006·Cell Division·Karen Crasta, Uttam Surana
Nov 24, 2006·BMC Evolutionary Biology·David A FitzpatrickGeraldine Butler
May 15, 2007·Cell Cycle·Nick R BrownLouise N Johnson
Apr 22, 2008·Nucleic Acids Research·A DereeperO Gascuel
Jun 4, 2008·Genes & Development·Rami Rahal, Angelika Amon
Jul 18, 2008·Nature·Jan M SkotheimFrederick R Cross
May 30, 2009·Molecular Biology of the Cell·Pierre CôteMalcolm Whiteway

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