Candida krusei produces ethanol without production of succinic acid; a potential advantage for ethanol recovery by pervaporation membrane separation

FEMS Yeast Research
Shunichi NakayamaDai Kitamoto

Abstract

The development of fermentative yeasts secreting no organic acids is highly desirable for ethanol production coupled with membrane separation processes, because the acidic byproduct, succinic acid, significantly inhibits the membrane permeation of ethanol. Of the Pichia and Candida yeasts tested, Candida krusei IA-1 showed the highest ethanol productivity [55 g L(-1) day(-1) from 150 g L(-1) (w/v) of glucose], comparable to the strains of Saccharomyces cerevisiae, and produced much less of the acid (0.6 g L(-1) day(-1)) than the Saccharomyces strains (1.5-1.8 g L(-1) day(-1)) under semi-aerobic conditions. Interestingly, under aerobic conditions, strain IA-1 showed no production of the acid. Stain IA-1 exhibited a good assimilation of the acid, while S. cerevisiae NBRC 0216 showed no assimilation. The activity of succinate dehydrogenase (SDH) in strain IA-1 was 37.5 mU mg(-1), and 7.8-fold higher than that in S. cerevisiae strain NBRC 0216. More significantly, SDH1 was abundantly transcribed in strain IA-1, different from that in strain NBRC 0216, regardless of the culture conditions. From these results, C. krusei IA-1 efficiently takes up succinic acid and metabolizes it in the Krebs cycle, producing an extremely low level of ...Continue Reading

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Citations

Jun 26, 2010·Applied Microbiology and Biotechnology·Churairat MoukamnerdYoshio Katakura
Jun 30, 2009·Journal of Industrial Microbiology & Biotechnology·Yan-Ning ZhengDong-Zhi He
Jul 25, 2012·Canadian Journal of Microbiology·Jay Shankar Singh YadavR Y Surampalli
May 12, 2012·Journal of Bioscience and Bioengineering·Shunichi NakayamaAtsumi Nakazato
Oct 25, 2011·Journal of Bioscience and Bioengineering·Naoto IsonoMakoto Hisamatsu
Mar 17, 2017·Microbial Cell Factories·Paulo Gonçalves TeixeiraJens Nielsen
May 14, 2020·Medical Mycology·A T JamiuC H Pohl
Jan 26, 2021·Biochemistry and Biophysics Reports·Asmamaw TesfawFassil Assefa

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