journal cover

Candida utilis NAD+ kinase: purification, properties and affinity gel studies

The International Journal of Biochemistry

Jan 1, 1982

J R Butler, E T McGuinness

PMID: 6290285

Abstract

1. NAD+ kinase (ATP:NAD+ 2' phosphotransferase, EC 2.7.1.23) has been purified to apparent enzymic homogeneity on Blue Sepharose CL-6B. 2. The molecular weight of the active species is about 260,000 as determined by PAGE and gel chromatography. Protein staining (PAGE) revealed minor ban...read more

Mentioned in this Paper

NAD+ kinase
Torulopsis utilis
SDS-PAGE
Gel Chromatography
Purification Aspects
Blue Sepharose CL-6B
Phosphotransferases
Ligands Activity
Hydrogen-Ion Concentration
Candida utilis
Paper Details
References
  • References14
  • Citations11
12
  • References14
  • Citations11
12

Related Papers

Biochimica Et Biophysica Acta

Isolation and characterization of yeast nicotinamide adenine dinucleotide kinase

Biochimica Et Biophysica ActaMay 10, 1979
Y M TsengM K Jacobson
© 2020 Meta ULC. All rights reserved

Candida utilis NAD+ kinase: purification, properties and affinity gel studies

The International Journal of Biochemistry

Jan 1, 1982

J R Butler, E T McGuinness

PMID: 6290285

DOI:

Abstract

1. NAD+ kinase (ATP:NAD+ 2' phosphotransferase, EC 2.7.1.23) has been purified to apparent enzymic homogeneity on Blue Sepharose CL-6B. 2. The molecular weight of the active species is about 260,000 as determined by PAGE and gel chromatography. Protein staining (PAGE) revealed minor ban...read more

Mentioned in this Paper

NAD+ kinase
Torulopsis utilis
SDS-PAGE
Gel Chromatography
Purification Aspects
Blue Sepharose CL-6B
Phosphotransferases
Ligands Activity
Hydrogen-Ion Concentration
Candida utilis

Related Papers

Biochimica Et Biophysica Acta

Isolation and characterization of yeast nicotinamide adenine dinucleotide kinase

Biochimica Et Biophysica ActaMay 10, 1979
Y M TsengM K Jacobson
Paper Details
References
  • References14
  • Citations11
12
  • References14
  • Citations11
12
/papers/candida-utilis-nad-kinase-purification-properties/6290285