Candidate Genes for Yellow Leaf Color in Common Wheat (Triticum aestivum L.) and Major Related Metabolic Pathways according to Transcriptome Profiling

International Journal of Molecular Sciences
Huiyu WuLingli Zhang

Abstract

The photosynthetic capacity and efficiency of a crop depends on the biosynthesis of photosynthetic pigments and chloroplast development. However, little is known about the molecular mechanisms of chloroplast development and chlorophyll (Chl) biosynthesis in common wheat because of its huge and complex genome. Ygm, a spontaneous yellow-green leaf color mutant of winter wheat, exhibits reduced Chl contents and abnormal chloroplast development. Thus, we searched for candidate genes associated with this phenotype. Comparative transcriptome profiling was performed using leaves from the yellow leaf color type (Y) and normal green color type (G) of the Ygm mutant progeny. We identified 1227 differentially expressed genes (DEGs) in Y compared with G (i.e., 689 upregulated genes and 538 downregulated genes). Gene ontology and pathway enrichment analyses indicated that the DEGs were involved in Chl biosynthesis (i.e., magnesium chelatase subunit H (CHLH) and protochlorophyllide oxidoreductase (POR) genes), carotenoid biosynthesis (i.e., β-carotene hydroxylase (BCH) genes), photosynthesis, and carbon fixation in photosynthetic organisms. We also identified heat shock protein (HSP) genes (sHSP, HSP70, HSP90, and DnaJ) and heat shock transc...Continue Reading

References

Jan 15, 1996·European Journal of Biochemistry·R D WillowsD von Wettstein
Jan 1, 1997·Cellular and Molecular Life Sciences : CMLS·L Nover, K D Scharf
Dec 10, 1997·Proceedings of the National Academy of Sciences of the United States of America·J KropatC F Beck
May 19, 1998·Cell Stress & Chaperones·M E Cheetham, A J Caplan
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·M K GreeneS J Landry
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·A HanssonM Hansson
Oct 25, 2000·Trends in Plant Science·K I TakamiyaH Ohta
Dec 15, 2000·The Journal of Biological Chemistry·I AdamskaK Kloppstech
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·N MochizukiJ Chory
Aug 10, 2001·Trends in Plant Science·P Albertsson
Oct 3, 2002·Proceedings of the National Academy of Sciences of the United States of America·A HanssonM Hansson
Feb 8, 2003·Science·Robert M LarkinJoanne Chory
Apr 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Claire HutinMichel Havaux
May 30, 2003·Plant & Cell Physiology·Ki-Hong JungGynheung An
May 8, 2004·Trends in Plant Science·Wangxia WangArie Altman
Feb 8, 2006·Plant Molecular Biology·Ruairidh J H SawersThomas P Brutnell
Apr 11, 2006·Current Opinion in Plant Biology·Ayumi Tanaka, Ryouichi Tanaka
May 4, 2006·Annual Review of Plant Biology·S Hörtensteiner
Jul 11, 2006·Plant Physiology·Mounia HeddadIwona Adamska
Jul 18, 2006·Nucleic Acids Research·Jia YeJun Wang
Jul 18, 2006·Nucleic Acids Research·Jianmin WuLiping Wei
Jun 8, 2007·The Plant Journal : for Cell and Molecular Biology·Tzvetelina Tzvetkova-ChevolleauMichel Havaux
Feb 26, 2008·Plant Physiology and Biochemistry : PPB·Inger Andersson, Anders Backlund
May 17, 2008·International Journal of Plant Genomics·Ana Conesa, Stefan Götz
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Aug 23, 2008·Journal of Proteomics·Anna Maria TimperioLello Zolla
Jan 17, 2009·Plant & Cell Physiology·Joonyul KimDean Dellapenna

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Citations

Oct 13, 2018·International Journal of Molecular Sciences·Bartolomé Sabater
Nov 17, 2019·International Journal of Molecular Sciences·Saeed Ul HaqZhen-Hui Gong

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Methods Mentioned

BETA
RNA-Seq
PCR
transgenic
ELIPs
ELIP
electrophoresis
cDNA library construction

Software Mentioned

GOseq R package
OmicShare
BLASTx
edgeR
Cuffmerge
FIMO
Blast2GO
Primer Premier
WEGO
Bowtie2

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