Candidate urinary biomarker discovery in ureteropelvic junction obstruction: a proteomic approach

The Journal of Urology
Hrair-George O MesrobianBassam Wakim

Abstract

Ureteropelvic junction obstruction may either worsen and require surgery, improve or remain stable. It may take upward of 3 years for the natural history to unfold. Urinary proteome analysis using capillary electrophoresis mass spectrometry has been shown to differentiate between normal infants and those with ureteropelvic junction obstruction. We sought to confirm these findings using liquid chromatography/nano-spray mass spectrometry to examine the urinary proteome in patients with unilateral grade IV ureteropelvic junction obstruction compared to age matched healthy infants. Urine specimens were obtained from 21 healthy infants with normal maternal/fetal ultrasound and 25 infants with grade IV unilateral ureteropelvic junction obstruction. Specimens were prepared using standard methods and subjected to liquid chromatography/tandem mass spectrometry analysis. Normalized data were annotated using the IPA(R) knowledge platform. There were 31 proteins significantly different in their level of abundance at 1 to 6 months, and 18 at 7 to 12 months compared to age matched controls. These proteins clustered into major functional networks. All of the biomarkers previously reported in clinical studies of ureteropelvic junction obstruct...Continue Reading

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Citations

Sep 26, 2013·Clinical & Developmental Immunology·Ana Cristina Simões e SilvaEduardo Araújo Oliveira
Feb 19, 2011·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Mariana A VasconcelosEduardo A Oliveira
Apr 23, 2015·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Ali SevenSuna Kabil Kucur
Aug 16, 2012·Scandinavian Journal of Urology·Lene StødkildeJørgen Frøkiær
Jul 24, 2012·The Journal of Urology·Bo Wu, James D Brooks
Jan 12, 2011·Journal of Pediatric Urology·Mia Gebauer MadsenTroels Munch Jørgensen
Aug 4, 2012·Pediatric Clinics of North America·Hrair-George O Mesrobian, Shama P Mirza
Nov 17, 2010·Experimental Eye Research·Rachida A BouhenniDeepak P Edward
Oct 10, 2015·Journal of the American Society of Nephrology : JASN·Florin L CraciunVishal S Vaidya
May 25, 2016·Molecular & Cellular Proteomics : MCP·John W FroehlichRichard S Lee
Aug 6, 2020·International Journal of Molecular Sciences·Satoshi WashinoTomoaki Miyagawa
Oct 30, 2016·Expert Review of Proteomics·Pedro MagalhãesPetra Zürbig

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