"Candidatus Similichlamydia laticola", a novel Chlamydia-like agent of epitheliocystis in seven consecutive cohorts of farmed Australian barramundi, Lates calcarifer (Bloch)

PloS One
Megan C StrideBarbara F Nowak

Abstract

Six consecutively hatched cohorts and one cohort of pre-hatch eggs of farmed barramundi (Lates calcarifer) from south Australia were examined for Chlamydia-like organisms associated with epitheliocystis. To identify and characterise the bacteria, 59 gill samples and three pre-hatch egg samples were processed for histology, in situ hybridisation and 16S rRNA amplification, sequencing and comprehensive phylogenetic analysis. Cases of epitheliocystis were observed microscopically and characterised by membrane-enclosed basophilic cysts filled with a granular material that caused hypertrophy of the epithelial cells. In situ hybridisation with a Chlamydiales-specific probe lead to specific labelling of the epitheliocystis inclusions within the gill epithelium. Two distinct but closely related 16S rRNA chlamydial sequences were amplified from gill DNA across the seven cohorts, including from pre-hatch eggs. These genotype sequences were found to be novel, sharing 97.1 - 97.5% similarity to the next closest 16S rRNA sequence, Ca. Similichlamydia latridicola, from Australian striped trumpeter. Comprehensive phylogenetic analysis of these genotype sequences against representative members of the Chlamydiales order and against other epithe...Continue Reading

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Citations

Jul 15, 2015·Journal of Applied Genetics·Małgorzata Pawlikowska-Warych, Wiesław Deptuła
Mar 23, 2018·Diseases of Aquatic Organisms·A SteigenØ Brevik
May 24, 2018·Transboundary and Emerging Diseases·Makeely Isabel BlandfordAdam Polkinghorne
Jul 1, 2020·International Journal of Systematic and Evolutionary Microbiology·Aharon OrenMartha E Trujillo
Dec 6, 2016·Frontiers in Microbiology·Alexander G J FehrLloyd Vaughan

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Datasets Mentioned

BETA
KF219613
KF219614

Methods Mentioned

BETA
PCR
light microscopy

Software Mentioned

jModelTest
BLAST
SPSS
CIPRES
Mr Bayes
MEGA5

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