Canine mast cell activation via human IgG1 and IgG4

International Archives of Allergy and Immunology
Yoshitaka SatoSeiichi Kitani

Abstract

We have reported that canine mastocytoma-derived CM-MC cells are activated via canine IgG and express a high-affinity IgG receptor (canine FcgammaRI). The predicted amino acid sequence of the canine FcgammaRI alpha subunit was found to be 72% similar to that of humans. These results suggest that canine FcgammaRI have binding activity with human IgG and led us to investigate CM-MC activation via canine FcgammaRI and human IgG. The binding of human IgG to canine FcgammaRI was examined by flow cytometry using FITC-conjugated human IgG. [Ca2+]i increase or histamine release via canine FcgammaRI and the four human IgG subclasses was measured following aggregation of IgG-bound FcgammaRIs by anti-human IgG. To determine the binding activity of canine FcgammaRI with human IgG1 or IgG3, the displacement of 125I-labeled canine IgG from canine FcgammaRI was examined by unlabeled human IgG1 or IgG3. The fluorescence intensity of CM-MC cells was markedly (about 50 times) elevated by incubation with FITC-human IgG compared with the fluorescence of the control cells. A significant (p < 0.01) calcium response and histamine release were observed following aggregation of canine FcgammaRIs bound with human IgG1 or IgG4. 125I-labeled canine IgG wa...Continue Reading

Citations

Dec 5, 2008·Veterinary Immunology and Immunopathology·Tzu-Yin LinCheryl A London
Jul 8, 2008·International Immunopharmacology·Hirofumi Nishikawa, Seiichi Kitani
Oct 10, 2006·Veterinary Immunology and Immunopathology·Tzu-Yin Lin, Cheryl A London
Sep 16, 2006·Veterinary Research·Bettina WagnerDouglas F Antczak

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