Canine oviductal exosomes improve oocyte development via EGFR/MAPK signaling pathway.

Reproduction : the Official Journal of the Society for the Study of Fertility
Seok Hee LeeByeong Chun Lee

Abstract

Oviduct cells produce a favorable environment for the development of gametes by generating multiple growth factors. Particularly, in canine species, immature oocytes undergo serial maturation processes in the oviduct, while the other mammals already possess matured oocytes in ovulatory follicles. However, little is known about the potential effect exhibited by the components released from canine oviduct cells (OCs) for modulating the biological function of oocytes. Recently, exosomes are regarded as promising extracellular vesicles because they represent considerable data for molecular cargo. Therefore, we first investigated the effect of canine oviductal exosomes (OC-Exo) on oocyte development via EGFR/MAPK pathway. Our results showed that OC-Exo labeled with PHK67 are successfully incorporated with cumulus cells and oocytes during IVM. Also, OC-Exo markedly increased the proportion of cumulus-oocyte complexes (COCs) exhibiting cumulus expansion as well as cumulus cell proliferation and maturation rate of oocytes (P < 0.05). Furthermore, gene expression patterns related with EGFR/MAPK pathway including EGFR, PKA, TACE/ADAM17, MAPK1/3, MAPK14, PTGS2, TNFAIP6, GDF9, and BMP15 were positively modified in COCs cultured with OC-Exo...Continue Reading

Citations

Oct 30, 2020·Zygote : the Biology of Gametes and Early Embryos·Daniel VeraguasLleretny Rodriguez-Alvarez
Nov 26, 2020·Animals : an Open Access Journal From MDPI·Maria G GervasiDavid Martín-Hidalgo
Oct 31, 2020·Animals : an Open Access Journal From MDPI·Seok Hee Lee, Islam M Saadeldin
Nov 11, 2020·International Journal of Molecular Sciences·Emily A HarrisWipawee Winuthayanon
Mar 7, 2021·Animals : an Open Access Journal From MDPI·Seok Hee LeeIslam M Saadeldin
Aug 8, 2021·Animals : an Open Access Journal From MDPI·Martina ColomboGaia Cecilia Luvoni

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