Canine parvovirus capsid structure, analyzed at 2.9 A resolution

Journal of Molecular Biology
Q Xie, M S Chapman

Abstract

The DNA-containing capsid of canine parvovirus (CPV) is analyzed following atomic refinement at 2.9 A resolution. The capsid contains 60 copies of the capsid protein related by icosahedral symmetry. The atomic model has been extended from the first residue (Gly37) of the unrefined 3.25 A structure towards the N terminus. The electron density shows that approximately 87% of the capsid proteins have N termini on the inside of the capsid, but for approximately 13%, the polypeptide starts on the outside and runs through one of the pores surrounding each 5-fold axis, explaining apparently conflicting antigenic data. Analysis of potential hydrogen bonds reveals approximately 50% more secondary structure than previously apparent. Most of the additional secondary structure are in the 71 and 221 residue-long loop insertions between beta-strands E and F and G and H, forming subunit-bridging sheets that likely add specificity to assembly interactions. Structural analysis of the extensive subunit interactions around the 3-fold axes shows that assembly is a multistep process with loops intertwining following initial contact. Estimated free energies of association suggest that the formation of 3 and 5-fold contacts likely takes precedence ov...Continue Reading

Citations

Oct 3, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kim Van VlietRichard O Snyder
Jul 31, 2013·Quarterly Reviews of Biophysics·Michael G Rossmann
Nov 30, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Sujata HalderMavis Agbandje-McKenna
May 30, 2008·Journal of Virology·Bärbel KaufmannMichael G Rossmann
Aug 15, 2008·Journal of Virology·Christian D S NelsonColin R Parrish
Aug 13, 2010·Journal of Virology·Bärbel KaufmannMichael G Rossmann
Aug 31, 2007·Journal of Virology·Hyun-Joo NamMavis Agbandje-McKenna
Sep 15, 2006·Journal of Virology·Lakshmanan GovindasamyMavis Agbandje-McKenna
Mar 12, 2010·Journal of Virology·Einari A NiskanenMaija Vihinen-Ranta
Mar 4, 2011·Journal of Virology·Bärbel KaufmannMichael G Rossmann
Mar 2, 2013·Journal of Virology·Sujata HalderMavis Agbandje-McKenna
Nov 25, 2011·Journal of Virology·Karla M StuckerColin R Parrish
Sep 20, 2002·Journal of Virology·Marty R BadgettJames J Bull
Sep 13, 2003·Journal of Virology·Sanna SuikkanenMaija Vihinen-Ranta
Jun 15, 2004·Journal of Virology·Maija Vihinen-RantaColin R Parrish
Mar 30, 2005·Journal of Virology·Eric PadronMavis Agbandje-McKenna
Apr 14, 2005·Journal of Virology·Stephanie KronenbergJürgen A Kleinschmidt
Jan 19, 1999·Annual Review of Microbiology·H Kasamatsu, A Nakanishi
Aug 4, 2004·Proceedings of the National Academy of Sciences of the United States of America·Bärbel KaufmannMichael G Rossmann
Jul 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Qing XieMichael S Chapman
Jul 9, 2016·The Journal of General Virology·Carla Miranda, Gertrude Thompson
Dec 17, 2014·Avian Pathology : Journal of the W.V.P.A·Bon-Sang KooIn-Pil Mo
Sep 4, 2015·Journal of Structural Biology·Sujata HalderMavis Agbandje-McKenna
Apr 5, 2001·Journal of Molecular Biology·S S KrishnaM R Murthy
Oct 16, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Joseph E Rabinowitz, R Jude Samulski
Dec 4, 2003·Journal of Molecular Biology·Charlotte HelgstrandLars Liljas
Mar 17, 2005·Journal of Molecular Biology·Anneke BruemmerElizabeth A Hewat
Mar 28, 2006·Biophysical Journal·Michael F Hagan, David Chandler
Oct 19, 2004·Journal of Virological Methods·Qing XieMichael S Chapman
Sep 12, 2014·Transboundary and Emerging Diseases·L YiS Cheng
Jan 27, 2017·Archives of Virology·Soulasack VannamahaxayPhongsakorn Chuammitri
Oct 5, 2017·Viruses·Elina MäntyläMaija Vihinen-Ranta
Sep 13, 2013·Journal of Virology·Geng MengMichael G Rossmann
Aug 19, 2016·Journal of Virology·Lindsey J OrgantiniSusan Hafenstein

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.