Canine visceral leishmaniasis diagnosis: a comparative performance of serological and molecular tests in symptomatic and asymptomatic dogs

Epidemiology and Infection
Flávio Luiz Nunes de CarvalhoMaria Lucia Rosa Rossetti

Abstract

Although serological assays have been widely used for the diagnosis of canine visceral leishmaniasis (CVL), they present different performances depending on the clinical profile of the dogs. This study evaluated the accuracy of serological tests, immunochromatographic (Dual Path Platform: DPP®) and enzyme-linked immunosorbent (ELISA EIE®), for CVL in relation to the detection of Leishmania DNA through real-time polymerase chain reaction (real-time PCR) in samples from symptomatic and asymptomatic dogs from a non-endemic area in the state of Rio Grande do Sul, Southern Brazil. Serum from 140 dogs (39 symptomatic and 101 asymptomatic) was tested by DPP and ELISA followed by real-time PCR. From a total of 140 samples evaluated, Leishmania DNA was detected by real-time PCR in 41.4% (58/140). Moreover, 67.2% of samples positive in real-time PCR were positive in both DPP and ELISA (39/58), showing moderate agreement between methods. In the symptomatic group, one sample non-reactive in both serological assays was positive in real-time PCR, whereas in the asymptomatic group, 17.8% non-reactive or undetermined samples in serological assays were positive in the molecular method. Leishmania DNA was not detected in 17.9% reactive samples b...Continue Reading

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Citations

Sep 13, 2019·PLoS Neglected Tropical Diseases·Ricardo Dias PortelaStella Maria Barrouin-Melo
Feb 7, 2019·Memórias do Instituto Oswaldo Cruz·Ana Izabel Passarella TeixeiraGustavo Adolfo Sierra Romero
Aug 7, 2019·PLoS Neglected Tropical Diseases·Monica E StaniekJames G C Hamilton
Aug 9, 2018·Parasitology Research·Juliana Barbosa NunesMarcos José Marques
Feb 7, 2019·Memórias do Instituto Oswaldo Cruz·Anna Raquel Ribeiro Dos SantosAna Paula Fernandes
Aug 8, 2019·Applied Microbiology and Biotechnology·Henrique Gama KerAlexandre Barbosa Reis
Sep 22, 2020·Parasitology·Iolanda Graepp-FontouraAna Lúcia Abreu-Silva
Dec 15, 2020·Journal of the American Veterinary Medical Association·Catherine G LammChristian M Leutenegger
Feb 9, 2021·Veterinary Parasitology·Úrsula Maira Russo ChagasCélia Maria Ferreira Gontijo
Jun 18, 2021·Frontiers in Plant Science·Bruno B SilvaMaria Izabel F Guedes
Sep 9, 2021·Revista Da Sociedade Brasileira De Medicina Tropical·Anaiá da Paixão SeváFernando Ferreira

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