Cannabinoid- and lysophosphatidylinositol-sensitive receptor GPR55 boosts neurotransmitter release at central synapses

Proceedings of the National Academy of Sciences of the United States of America
Sergiy SylantyevDmitri A Rusakov

Abstract

G protein-coupled receptor (GPR) 55 is sensitive to certain cannabinoids, it is expressed in the brain and, in cell cultures, it triggers mobilization of intracellular Ca(2+). However, the adaptive neurobiological significance of GPR55 remains unknown. Here, we use acute hippocampal slices and combine two-photon excitation Ca(2+) imaging in presynaptic axonal boutons with optical quantal analysis in postsynaptic dendritic spines to find that GPR55 activation transiently increases release probability at individual CA3-CA1 synapses. The underlying mechanism involves Ca(2+) release from presynaptic Ca(2+) stores, whereas postsynaptic stores (activated by spot-uncaging of inositol 1,4,5-trisphosphate) remain unaffected by GPR55 agonists. These effects are abolished by genetic deletion of GPR55 or by the GPR55 antagonist cannabidiol, a constituent of Cannabis sativa. GPR55 shows colocalization with synaptic vesicle protein vesicular glutamate transporter 1 in stratum radiatum. Short-term potentiation of CA3-CA1 transmission after a short train of stimuli reveals a presynaptic, Ca(2+) store-dependent component sensitive to cannabidiol. The underlying cascade involves synthesis of phospholipids, likely in the presynaptic cell, but not...Continue Reading

References

Apr 27, 2002·Science·Rachel I Wilson, Roger A Nicoll
Jun 11, 2002·Nature Neuroscience·Thomas G OertnerKarel Svoboda
Jun 30, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ricardo Scott, Dmitri A Rusakov
Oct 25, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jill B Jensen, Jane E Lauckner
Sep 4, 2007·Biochemical and Biophysical Research Communications·Saori OkaTakayuki Sugiura
Sep 19, 2007·British Journal of Pharmacology·E RybergP J Greasley
Feb 12, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jane E LaucknerKen Mackie
Sep 2, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christopher M HenstridgeAndrew J Irving
Sep 9, 2008·Nature Medicine·István Katona, Tamás F Freund
Dec 5, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ricardo ScottDmitri A Rusakov
Feb 21, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Duncan RyanBettina Platt
Feb 24, 2009·Trends in Pharmacological Sciences·Ruth A Ross
Nov 20, 2009·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Sagnik BhattacharyyaPhilip K McGuire
Dec 2, 2009·The British Journal of Psychiatry : the Journal of Mental Science·Marta Di FortiRobin M Murray
Jan 16, 2010·Nature·Christian HennebergerDmitri A Rusakov
Oct 5, 2010·The British Journal of Psychiatry : the Journal of Mental Science·Celia J A MorganH Valerie Curran
Feb 1, 2011·Current Opinion in Neurobiology·Alexander Dityatev, Dmitri A Rusakov
Mar 2, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Fritz KobeEvgeni Ponimaskin

❮ Previous
Next ❯

Citations

Nov 19, 2013·PloS One·Joseph BouskilaJean-François Bouchard
Apr 22, 2014·Journal of Clinical Psychopharmacology·Tibor M BruntRaymond J M Niesink
Jun 17, 2014·Nutrition Research Reviews·Gerry Jager, Renger F Witkamp
Jun 28, 2014·Pharmacology & Therapeutics·Bo LiuShanta J Persaud
Mar 5, 2016·Nature Reviews. Drug Discovery·Mark J MillanDaniel Weinberger
Nov 18, 2015·European Journal of Medicinal Chemistry·Sari YrjöläTapio J Nevalainen
Jun 9, 2014·Neuron·Angela Maria VergnanoPierre Paoletti
Aug 19, 2015·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Evan C RosenbergOrrin Devinsky
Aug 13, 2015·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Clementino Ibeas BihBenjamin J Whalley
Aug 4, 2016·Visual Neuroscience·Joseph BouskilaMaurice Ptito
Sep 10, 2015·The New England Journal of Medicine·Daniel Friedman, Orrin Devinsky
May 7, 2013·Behavioural Brain Research·Fabricio H Do MonteReinaldo N Takahashi
Dec 3, 2014·Medical Hypotheses·Maurizio Coppola, Raffaella Mondola
Aug 27, 2016·Diabetes, Obesity & Metabolism·Bo LiuShanta J Persaud
Nov 26, 2016·Nature Communications·Kim BoddumMatthew C Walker
Apr 11, 2017·Neuropharmacology·Carl R LupicaAlexander F Hoffman
Oct 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Joshua S KaplanRuth E Westenbroek
Jun 12, 2018·British Journal of Pharmacology·Jeremy D HillYuri Persidsky
Jan 25, 2019·Current Opinion in Neurology·Santoshi BillakotaEric Marsh
Feb 23, 2018·Nature Protocols·Kaiyu ZhengDmitri A Rusakov
Dec 14, 2019·Nature Reviews. Neurology·Luigia CristinoVincenzo Di Marzo
Dec 29, 2019·Antioxidants·Sinemyiz AtalayElzbieta Skrzydlewska
Sep 5, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Falone Sunda, Afolake Arowolo
Feb 16, 2017·Journal of Molecular Endocrinology·Eva TuduríRubén Nogueiras
Apr 13, 2018·Epilepsy Currents·Akshay Gupta, Viji Santhakumar

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.