Nov 5, 2018

Whole cell response to receptor stimulation involves many deep and distributed subcellular processes

BioRxiv : the Preprint Server for Biology
Jens HansenRavi Iyengar


Neurite outgrowth is an integrated whole cell response regulated by cannabinoid-1 receptor. To understand underlying mechanisms, we identified subcellular processes (SCPs) and their interactions required for the response. Differentially expressed genes and proteins upon receptor stimulation of neuronal cells were used informatically to build networks of SCPs and their interactions. From SCP networks we identified additional genes, which when ablated validated the SCP involvement in neurite outgrowth. The experiments and informatics analyses identified diverse SCPs such as those involved in pyrimidine metabolism, lipid biosynthesis, mRNA splicing and stability along with membrane vesicle and microtubule dynamics. We find that SCPs required for neurite outgrowth are widely distributed among constitutive cellular functions. Several of these SCPs are deep since they are distal to cell signaling pathways and proximal SCPs involved in microtubule growth and membrane vesicle dynamics. We conclude that receptor regulation of SCPs for neurite outgrowth is distributed and deep.

  • References
  • Citations


  • We're still populating references for this paper, please check back later.
  • References
  • Citations


  • This paper may not have been cited yet.

Mentioned in this Paper

Biochemical Pathway
Regulation of Biological Process
Neurite Outgrowth
Cell Process Structure
Cerebral Palsy, Spastic
Pyrimidine Metabolism Pathway

About this Paper

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

© 2020 Meta ULC. All rights reserved