Cannabinoid CB1 receptor-mediated inhibition of glutamate release from rat hippocampal synaptosomes.

European Journal of Pharmacology
Su-Jane Wang

Abstract

Cannabinoid receptors are widely expressed in the brain and have been shown to regulate synaptic transmission through a presynaptic mechanism. Using synaptosomal preparation, I show here that 2,3-dihydro-5-methyl-3-(4-morpholinyl-methyl)-pyrrolo-1,4-benzoxazin-6-yl-1-naphthalenylmethanone (WIN 55212-2) strongly depressed 4-aminopyridine-evoked glutamate release in a concentration-dependent manner, and this effect was reversed by the selective cannabinoid CB(1) receptor antagonist 1-(2,4-dichlorophenyl)-5-(4-iodophenyl)-4-methyl-N-4-morpholinyl-1H-pyrazole-3-carboxamide (AM 281). The inhibitory modulation by WIN 55212-2 was not due to a decrease in synaptosomal excitability or a direct effect on the release machinery because WIN 55212-2 did not alter 4-aminopyridine-mediated depolarization and ionomycin-induced glutamate release. In addition, the WIN 55212-2-mediated inhibition of glutamate release was blocked by the G(i)/G(o) protein inhibitor pertussis toxin, but not by the protein kinase A inhibitor 2,3,9,10,11,12-Hexahydro-10-hydroxy-9-methyl-1-oxo-9,12-epoxy-1H-diindolo-benzodiazocine-10-carboxylic acid, hexyl ester (KT 5720). Furthermore, this inhibitory effect was associated with a decrease in 4-aminopyridine-evoked Ca(2+...Continue Reading

References

Sep 1, 1990·Trends in Pharmacological Sciences·B Meldrum, J Garthwaite
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M HerkenhamK C Rice
Jun 19, 1986·Nature·D G Nicholls, T S Sihra
Jan 1, 1995·Annual Review of Pharmacology and Toxicology·A C Howlett
Jun 1, 1996·Trends in Neurosciences·J Sánchez-PrietoD G Nicholls
Sep 27, 1996·Biochemical Pharmacology·S R Childers, S A Deadwyler
May 1, 1997·Trends in Neurosciences·L G Wu, P Saggau
Jul 1, 1997·Journal of Neurophysiology·W TwitchellK Mackie
Jan 1, 1997·Pharmacology & Therapeutics·R G Pertwee
Jun 15, 1999·Progress in Neurobiology·A Ameri
Jun 6, 2000·Trends in Pharmacological Sciences·D PiomelliF Rodríguez de Fonseca

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Citations

Feb 16, 2012·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Deepak Cyril D'SouzaDaniel Mathalon
Mar 15, 2015·International Journal of Molecular Sciences·Tzu-Yu LinSu-Jane Wang
Nov 17, 2011·Neurochemistry International·João M N DuarteAttila Köfalvi
Sep 11, 2007·British Journal of Pharmacology·V A Campbell, A Gowran
Nov 1, 2013·British Journal of Pharmacology·S G Fagan, V A Campbell
Aug 21, 2007·Biochemical Pharmacology·Justin T Gass, M Foster Olive
Dec 9, 2009·Neurotoxicology·C PopeL Parsons
Sep 7, 2016·European Neuropsychopharmacology : the Journal of the European College of Neuropsychopharmacology·Gabriela B L SpiacciLeonardo B Resstel
Jun 15, 2016·Journal of Psychopharmacology·Mohammad NasehiMohammad-Reza Zarrindast

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