PMID: 11340650May 8, 2001Paper

Cannabinoid receptor agonist-stimulated [35S]guanosine triphosphate gammaS binding in the brain of C57BL/6 and DBA/2 mice

Journal of Neuroscience Research
Balapal S Basavarajappa, Basalingappa L Hungund

Abstract

The two inbred strains of mice C57BL/6 (alcohol-preferring) and DBA/2 (alcohol-avoiding) mice have been shown to differ significantly in their preference for alcohol (EtOH). We have previously demonstrated the differences in the density and the affinity of cannabinoid (CB1) receptors in the brains of the two inbred C57BL/6 and DBA/2 mouse strains. In the present study, we investigated the CB1 receptor agonist-stimulated guanosine-5'-O-(3-[(35)S]thio)-triphosphate ([(35)S]GTPgammaS) binding in plasma membranes (PM) from C57BL/6 and DBA/2 mice. The results indicate that the net CP55,940-stimulated [(35)S]GTPgammaS binding was increased with increasing concentrations of CB1 receptor agonists and GDP. The net CB1 receptor agonist (WIN55,212-2 or HU-210 or CP55,940)-stimulated [(35)S]GTPgammaS binding was reduced significantly (-10% to -12%, P < 0.05) in PM from DBA/2 mice; no significant differences were observed in basal [(35)S]GTPgammaS binding among these strains. Nonlinear regression analysis of net CP55,940-stimulated [(35)S]GTPgammaS binding showed that the B(max) of cannabinoid agonist-stimulated binding was significantly reduced (-24%) in DBA/2 mice (B(max) = 12.43 +/- 0.64 for C57BL/6 and 9.46 +/- 0.98 pmol/mg protein for ...Continue Reading

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Citations

Jun 26, 2003·Trends in Pharmacological Sciences·Raphael Mechoulam, Linda Parker
Mar 31, 2006·Behavioural Pharmacology·Brett C Ginsburg, Richard J Lamb
May 1, 2002·Alcoholism, Clinical and Experimental Research·Basalingappa L HungundGeorge F Koob
Apr 12, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shivakumar SubbannaBalapal S Basavarajappa
Mar 14, 2002·Biochemical and Biophysical Research Communications·Krisztina MonoryJacques Hanoune
Jan 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Lei WangGeorge Kunos
Sep 22, 2006·Journal of Neurochemistry·Sabah KelaïLaurence Lanfumey
Feb 2, 2006·Neuropharmacology·Balapal S BasavarajappaBasalingappa L Hungund
Jul 12, 2005·Life Sciences·K Yaragudri Vinod, Basalingappa L Hungund
Nov 20, 2004·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Balapal S Basavarajappa, Basalingappa L Hungund
Nov 20, 2004·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Jorge ManzanaresTomás Palomo
Oct 21, 2010·Alcoholism, Clinical and Experimental Research·Panayotis K ThanosNora D Volkow
Oct 12, 2005·Alcohol and Alcoholism : International Journal of the Medical Council on Alcoholism·Karen L NowakBasalingappa L Hungund
Jul 3, 2019·British Journal of Pharmacology·Balapal S BasavarajappaShivakumar Subbanna
Nov 5, 2004·The Journal of Pharmacology and Experimental Therapeutics·Bernard Le Foll, Steven R Goldberg
Nov 16, 2004·Annals of the New York Academy of Sciences·Basalingappa L Hungund, Balapal S Basavarajappa
Dec 2, 2017·Pharmacology & Therapeutics·Mohammed AkbarAntonio Noronha
Jun 8, 2002·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Beat Lutz
Jun 8, 2002·Prostaglandins, Leukotrienes, and Essential Fatty Acids·B S Basavarajappa, B L Hungund

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