Canolol inhibits gastric tumors initiation and progression through COX-2/PGE2 pathway in K19-C2mE transgenic mice

PloS One
Donghui CaoXue-Yuan Cao

Abstract

4-Vinyl-2, 6-dimethoxyphenol (canolol) is an antioxidant phenolic compound extracted from crude canola oil. In current research, K19-C2mE transgenic mice, developing hyperplastic tumors spontaneously in the glandular stomach, were used to study the mechanisms involved in the anti-inflammation and anti-tumor effects of canolol. Tg mice receiving canolol diet had a reduced tumor incidence, to 41.2%, compared with Non-treatment Tg mice, 77.8% of which had gastric tumor (P=0.002). Besides that, the mean tumor diameter was decreased from 6.5 mm to 4.5 mm (P<0.001) after canolol administration. COX-2/PGE2 pathway is known to play pivotal role in inflammation-induced gastric tumorigenesis. The neutrophils and lymphocytes infiltration was suppressed significantly, and the mRNA levels of the proinflammatory cytokines COX-2, IL-1β and IL-12b were also downregulated in gastric mucosa. Additionally, immunohistochemical analysis showed that COX-2, EP2, Gαs and β-catenin, key factors involving in PGE2 signal transduction, were positive staining with higher H scores in Non-treatment Tg mice, while the expressions were suppressed significantly by 0.1% canolol (P<0.001). In addition, tumor-suppressor miR-7 was reactivated after canolol administ...Continue Reading

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Citations

Aug 27, 2015·Journal of Clinical Medicine·Jessica L HorshamPeter J Leedman
Aug 5, 2017·International Journal of Molecular Sciences·Tetsuya TsukamotoXueyuan Cao
May 18, 2019·Advances in Nutrition·McKale Montgomery, Aishwarya Srinivasan
Mar 21, 2019·Lipids·Klara KraljićKristina Radošević
Jun 1, 2021·Gastrointestinal Endoscopy Clinics of North America·Shailja C Shah, Richard M Peek

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Methods Mentioned

BETA
transgenic
nuclear translocation
genotyping
PCR
chips
transfection

Software Mentioned

SPSS
TargetScan

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