PMID: 11312252Apr 20, 2001Paper

Canonical germinal center B cells may not dominate the memory response to antigenic challenge

International Immunology
Y F LuJ Cerny

Abstract

Spleen and bone marrow (BM) are the major sites of antibody production and anamnestic response in systemically immunized mice. We examined the VDJ segment repertoire of antibody plaque-forming cells (APFC) in those two sites in the course of antibody responses to the hapten nitrophenyl (NP). Individual IgG APFC expressed any one of 10 V(H) segments of the V186.2/V3 (J558) gene family: 186.2, 102, 23, C1H4, 165.l, CH10, 3, 593.3, 24.8 and 671.5. The majority of cells in both spleen and BM expressed the V186.2 gene joined to a D segment with Tyr95. During a 2-month period after a single immunization, the V186.2(+) APFC in BM accumulated 3 times as many somatic mutations than splenic APFC (average 8.5 versus 3 mutations/V(H)); this process was T(h) dependent as shown by in vivo depletion of CD4(+) lymphocytes. However, the V186.2(+) APFC in both spleen and BM shared a recurrent W33L replacement, indicating their common origin from germinal centers. The APFC expressing the other (analogue) V(H) segments were evenly represented in the spleen and BM, but they accumulated few, if any, mutations. The anamnestic V186.2(+) APFC were highly mutated both in the spleen and BM; they represented a new and unexpected clonotype. The V/D segment...Continue Reading

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Citations

Jun 5, 2003·International Journal for Parasitology·David Scott McVeySteven C Olson
Jun 27, 2007·Molecular and Cellular Biology·Li BaiDemin Wang
Dec 25, 2013·The Journal of Experimental Medicine·Radhika GoenkaMichael P Cancro
Jan 7, 2014·Experimental Gerontology·Radhika GoenkaMichael P Cancro
Mar 3, 2004·European Journal of Immunology·Shuhua HanBiao Zheng
Sep 10, 2014·Viruses·Martina SoldemoGunilla B Karlsson Hedestam
Jul 18, 2019·The Journal of Experimental Medicine·Emily K MoserPaula M Oliver
Sep 6, 2020·International Immunology·Akiko Sugimoto-IshigeToshitada Takemori
Oct 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yi-Feng Lu, Jan Cerny

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