Capacitation-associated changes in membrane fluidity in asthenozoospermic human spermatozoa

International Journal of Andrology
M G BuffoneS V Verstraeten

Abstract

The fertilizing potential of human spermatozoa relies on their ability to capacitate as they travel through the female reproductive tract. During this process, cholesterol is released from the plasma membrane, altering its architecture and dynamics. Using ISolate gradients, we obtained high (L90)- and low (L45)-quality spermatozoa from asthenozoospermic human semen samples. We tested the hypothesis that the lower fertilizing ability of asthenozoospermic L90 cells could be related to a lower ability to increase their membrane fluidity during capacitation. We assessed two sets of fluorescent probes: (i) DPH, TMA-DPH and PA-DPH which senses the hydrophobic core, cytosolic and exofacial leaflets of the bilayer, respectively and (ii) Laurdan, sensitive to the amount of water molecules intercalated between lipid moieties of the membrane (membrane hydration). Before capacitation, membrane fluidity of asthenozoospermic sperm populations was similar to the corresponding fractions of normozoospermic cells when evaluated with DPH, TMA-DPH or PA-DPH. Asthenozoospermic whole samples displayed lower plasma membrane hydration than normozoospermic cells as evidenced with Laurdan. After capacitation, asthenozoospermic L45 and L90 cells failed t...Continue Reading

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Citations

Nov 14, 2013·Journal of Assisted Reproduction and Genetics·Florenza A La SpinaMariano G Buffone
Nov 24, 2012·Fertility and Sterility·Eleonora SalvoliniGiancarlo Balercia
May 11, 2013·Molecular Reproduction and Development·Wutigri NimlamoolLinda J Lowe-Krentz
Dec 31, 2011·Systems Biology in Reproductive Medicine·Elena MorettiGiulia Collodel
Jan 29, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Gustavo CélizSandra V Verstraeten
Jun 29, 2018·Journal of Cellular Physiology·Guillermina M LuqueMariano G Buffone
Aug 15, 2018·Frontiers in Cell and Developmental Biology·Lis C Puga MolinaMariano G Buffone
Jul 28, 2020·Molecular and Cellular Endocrinology·Maya FinkelsteinHaim Breitbart
Apr 26, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Nozomi WatanabeHiroshi Umakoshi

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