Capacitation status and in vitro fertility of boar spermatozoa: effects of seminal plasma, cumulus-oocyte-complexes-conditioned medium and hyaluronan

International Journal of Andrology
K SuzukiHeriberto Rodriguez-Martinez

Abstract

In the present study, the effects of seminal plasma (SP), cumulus-oocyte-complexes (COCs) conditioned medium (CCM) and hyaluronan (HA) on functional changes and in vitro fertilizing ability of porcine spermatozoa were examined. In in vitro fertilization (IVF) experiments, 10% (v/v) of exogenous SP in the fertilization medium prevented sperm penetration (using fresh-extended and frozen-thawed ejaculated spermatozoa). Analysis of frozen-thawed CCM revealed a HA content to levels of 30 ng/mL per incubated COC. Presence of frozen-thawed CCM did not, however, prove effective to increase (furthermore decreasing) oocyte penetration in vitro, and neither did supplementation with exogenous HA at the same concentration as that present in the CCM (secreted by COCs). Analysis of sperm capacitation using the chlortetracycline (CTC) assay showed that frozen-thawed CCM had no elevating effect on 'B-pattern' spermatozoa (implying capacitation-like changes) and that addition of 10% (v/v) SP held spermatozoa in the 'F-pattern' (intact) status. Dose of 500 microg/mL HA and freshly prepared CCM increased, however, the frequency of capacitated spermatozoa (B-pattern) without resulting in increased rates of 'AR-pattern' (acrosome-reacted) spermatozo...Continue Reading

References

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Citations

Mar 24, 2004·Animal Reproduction Science·P TienthaiH Rodriguez-Martinez
Aug 5, 2003·Reproduction, Fertility, and Development·Paisan TienthaiHeriberto Rodriguez-Martinez
Apr 23, 2005·The Journal of Reproduction and Development·Ann-Sofi BergqvistHeriberto Rodríguez-Martínez
Nov 9, 2005·The Journal of Reproduction and Development·Kazuhiro KikuchiNaomi Kashiwazaki
Oct 17, 2007·The Journal of Reproduction and Development·Mayuko KuromeHiroshi Nagashima
Nov 21, 2007·The Journal of Reproduction and Development·Carlos Manuel Ulloa UlloKazuhiro Kikuchi
Feb 3, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Hiroyuki KanekoNaomi Kashiwazaki
Mar 4, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Michiko NakaiKazuhiro Kikuchi
May 26, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Michiko NakaiKazuhiro Kikuchi
Oct 4, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Tamara Leahy, Bart M Gadella
Aug 2, 2011·Theriogenology·Melissa L Vadnais, Gary C Althouse
Dec 1, 2009·Animal Reproduction Science·J C GarciaR N Kirkwood
Aug 30, 2008·Theriogenology·R N KirkwoodM Abad
Aug 5, 2008·Theriogenology·M A GilE A Martínez
Dec 17, 2008·Reproduction in Domestic Animals = Zuchthygiene·D RathU Taylor
May 28, 2011·Animal Science Journal = Nihon Chikusan Gakkaihō·Thanh Quang Dang-NguyenTakashi Nagai
Jan 24, 2014·Animal Science Journal = Nihon Chikusan Gakkaihō·Fuminori TaniharaKazuhiro Kikuchi
Feb 11, 2014·Animal Science Journal = Nihon Chikusan Gakkaihō·Thanh Quang Dang-NguyenBui Xuan Nguyen
Jan 16, 2009·Reproduction in Domestic Animals = Zuchthygiene·N MaedomariN Kashiwazaki
Jul 20, 2013·Animal Science Journal = Nihon Chikusan Gakkaihō·Koji MisumiKazuhiro Kikuchi

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