PMID: 8594545Oct 1, 1995Paper

Capacitative Ca2+ entry contributes to the Ca2+ influx induced by thyrotropin-releasing hormone (TRH) in GH3 pituitary cells

Pflügers Archiv : European journal of physiology
C Villalobos, J Garcia-Sancho

Abstract

Treatment of GH3 cells with either hypothalamic peptide thyrotropin-releasing hormone (TRH), the endomembrane Ca2+-ATPase inhibitor thapsigargin or the Ca2+ ionophore ionomycin mobilized, with different kinetics, essentially all of the Ca2+ pool from the intracellular Ca2+ stores. Any of the above- described treatments induced a sustained increase in intracellular Ca2+ concentration ([Ca2+]i), which was dependent on extracellular Ca2+ and was prevented by Ni2+ but not by dihydropyridines (DHPs), suggesting that it was due to capacitative Ca2+ entry via activation of a plasma membrane pathway which opened upon the emptying of the intracellular Ca2+ stores. The increase of the plasma membrane permeability to Ca2+ correlated negatively with the filling degree of the intracellular Ca2+ stores and was reversed by refilling of the stores. The mechanism of capacitative Ca2+ entry into GH3 cells differed from similar mechanisms described in several types of blood cells in that the pathway was poorly permeable to Mn2+ and not sensitive to cytochrome P450 inhibitors. In GH3 cells, TRH induced a transient [Ca2+]i increase due to Ca2+ release from the stores (phase 1) followed by a sustained [Ca2+]i increase due to Ca2+ entry (phase 2). At...Continue Reading

References

May 15, 1992·The Biochemical Journal·J LlopisS Orrenius
Jun 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C VillalobosJ García-Sancho
Jan 6, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J AlvarezJ Garcia-Sancho
Aug 1, 1991·Trends in Pharmacological Sciences·J MeldolesiT Pozzan
Jan 1, 1986·The Journal of General Physiology·D R Matteson, C M Armstrong
Oct 1, 1988·Trends in Neurosciences·R W TsienA P Fox
Jan 1, 1989·Annual Review of Physiology·J R Williamson, J R Monck
Dec 1, 1989·Biochemical Society Transactions·J Garcia-SanchoA Sanchez
Jan 1, 1986·Annual Review of Physiology·M C Gershengorn
Sep 1, 1993·Pflügers Archiv : European journal of physiology·M MonteroJ García-Sancho
Oct 1, 1993·Endocrine Reviews·J W Putney, G S Bird

❮ Previous
Next ❯

Citations

Jan 30, 2010·Pflügers Archiv : European journal of physiology·Stefan Feske
Aug 12, 2003·Cell Calcium·James W Putney
Aug 15, 2000·Molecular and Cellular Endocrinology·A González IglesiasD Becu-Villalobos
Dec 1, 1996·Trends in Endocrinology and Metabolism : TEM·P M HinkleR Ashworth
Aug 4, 2006·Journal of Neurophysiology·Babak A KachoeiNeil S Magoski
Jul 24, 2010·Endocrine Reviews·Stanko S StojilkovicRichard Bertram
Feb 3, 2016·European Journal of Nutrition·Celia CarrilloSara R Alonso-Torre
Dec 13, 2005·The Journal of Physiology·Lucía NúñezCarlos Villalobos
Jul 15, 1997·The Journal of Physiology·C VillalobosJ García-Sancho
Mar 25, 2005·Physiological Reviews·Anant B Parekh, James W Putney
Jan 30, 2009·American Journal of Physiology. Cell Physiology·Hidetada MatsuokaMasumi Inoue
Mar 7, 2002·American Journal of Physiology. Cell Physiology·Rosalba I FonterizJavier García-Sancho
Nov 14, 1997·The Journal of Biological Chemistry·A LachowiczS S Stojilkovic
Aug 24, 2002·Endocrinology·Carlos VillalobosL Stephen Frawley
Jul 18, 2000·American Journal of Physiology. Cell Physiology·I VicarioC González
Jan 22, 1998·The American Journal of Physiology·C VillalobosJ García-Sancho
Jun 5, 1998·The American Journal of Physiology·L Nuñez, L S Frawley
Sep 14, 1999·The American Journal of Physiology·A G IglesiasD Becu-Villalobos
Aug 10, 2020·Mini Reviews in Medicinal Chemistry·Amir R AfshariAmirhossein Sahebkar

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.