Capacitative Ca2+ entry is involved in cAMP synthesis in mouse parotid acini

The American Journal of Physiology
E L WatsonS M Ott

Abstract

Muscarinic receptor interaction leading to augmentation of isoproterenol-stimulated cAMP accumulation in mouse parotid acini involves Ca2+ (28). The effectiveness of capacitative Ca2+ entry and intracellular Ca2+ release on this response was determined in time course studies by using three independent tools to manipulate the free intracellular Ca2+ concentration: the muscarinic agonist carbachol, thapsigargin, and ionomycin. Time course studies revealed that Ca2+ release from intracellular stores by carbachol produced an early rapid increase (0.25-0.5 min) in stimulated cAMP levels, whereas capacitative Ca2+ entry resulted in a sustained increase in stimulated cAMP levels that was blocked by La3+. Capacitative Ca2+ entry, alone, was involved in thapsigargin and ionomycin augmentation of stimulated cAMP accumulation. The inability of phosphodiesterase inhibitors, 3-isobutyl-1-methylxanthine and milrinone, to prevent agonist augmentation of cAMP levels, as well as the finding that the type VIII adenylyl cyclase (ACVIII) is expressed in parotid acini, suggests that capacitative Ca2+ entry augments stimulated cAMP accumulation, at least in part, via activation of this adenylyl cyclase isoenzyme.

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Citations

Oct 26, 1999·The Journal of Biological Chemistry·T J Shuttleworth, J L Thompson
Jul 7, 2007·Physiological Reviews·Debbie Willoughby, Dermot M F Cooper
Jan 21, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Adel H SaadYoshinori Marunaka
Apr 19, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Takashi NakahariYusuke Imai
Jul 27, 2004·Cerebrovascular Diseases·Hanne ChristensenGudrun Boysen
May 25, 2010·The Journal of Physiological Sciences : JPS·Hideyo YoshidaTakashi Nakahari
Aug 13, 1999·The American Journal of Physiology·E L WatsonS M Ott
Nov 7, 2008·American Journal of Physiology. Cell Physiology·Tomoyuki Saino, Eileen L Watson
Dec 18, 2009·Journal of Cell Science·Debbie WilloughbyDermot M F Cooper
Apr 15, 2000·Frontiers in Neuroendocrinology·F A Antoni
Feb 9, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·A ImaiH Shimomura
Nov 26, 2003·Cellular Signalling·Eileen L WatsonDennis H DiJulio
Mar 15, 2005·Cellular Signalling·Tasmina A Goraya, Dermot M F Cooper

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