PMID: 8594544Oct 1, 1995Paper

Capacitative Ca2+ influx and a Ca2+-dependent nonselective cation pathway are discriminated by genistein in mouse pancreatic acinar cells

Pflügers Archiv : European journal of physiology
F PfeifferI Schulz

Abstract

We have investigated the effect of genistein on the hormone-stimulated Ca2+ influx and on a 28pS nonselective cation channel in mouse pancreatic acinar cells using the Ca2+ indicator fluo3 and the patch-clamp technique. The identity of the Ca2+ influx pathway has not been established in this cell type so far. Therefore we have investigated the Ca2+-dependent nonselective cation channel as a potential pathway for Ca2+ influx. Capacitative Ca2+ entry was induced by depletion of intracellular Ca2+ stores with 500nM acetylcholine or with the Ca2+ ATPase inhibitor 2,5di-tert- butylhydroquinone. In the presence of 100microM genistein, Ca2+ release was unimpaired, whereas Ca2+ influx was reversibly suppressed. Patch-clamp experiments demonstrated that genistein had no effect on Ca2+-activated nonselective cation channels, the activity of which was measured in excised membrane patches (inside/out) or in the whole-cell configuration. Therefore we conclude that this 28pS nonselective cation channel does not contribute to Ca2+ influx into mouse exocrine pancreatic cells. With the exception of genistein and tyrphostin 25, other tyrosine kinase inhibitors such as methyl-2,5-dihydroxycinnamate, lavendustin A, herbimycin A, and tyrphostin B56...Continue Reading

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Citations

Oct 25, 2003·Cellular Signalling·Shyuefang HsuIrene Schulz
Jun 28, 2002·Physiological Reviews·Michael C Ashby, Alexei V Tepikin
Oct 23, 2001·American Journal of Physiology. Heart and Circulatory Physiology·M J DavisE Wilson
Apr 8, 1998·The American Journal of Physiology·F PfeifferI Schulz
Sep 9, 1998·The American Journal of Physiology·A GonzálezI Schulz

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