Capacitively coupled contactless conductivity detection as an alternative detection mode in CE for the analysis of kanamycin sulphate and its related substances

Journal of Separation Science
Mohamed N El-AttugAnn Van Schepdael

Abstract

A method was developed to determine simultaneously kanamycin, its related substances and sulphate in kanamycin sulphate using capacitively coupled contactless conductivity detection. Kanamycin is an aminoglycoside antibiotic that lacks a strong UV-absorbing chromophore. Due to its physicochemical properties, CE in combination with capacitively coupled contactless conductivity detection was chosen. The separation method uses a BGE composed of 40 mM 2-(N-morpholino)ethanesulphonic acid monohydrate and 40 mM L-histidine, pH 6.35. A 0.6 mM N-cetyltrimethyl ammonium bromide (CTAB) solution was added as electroosmotic flow modifier in a concentration below the critical micellar concentration (CMC). Ammonium acetate 50 mg/L was used as internal standard. In total, 30 kV was applied in reverse polarity on a fused-silica capillary (65/41 cm; 75 μm id). The optimized separation was obtained in less than 6 min with good linearity (R(2)=0.9999) for kanamycin. It shows a good precision expressed as RSD on the relative peak areas equal to 0.3 and 1.1% for intra-day and inter-day precision, respectively. The LOD and LOQ are 0.7 and 2.3 mg/L, respectively. Similarly, for sulphate, a good linearity (R(2)=0.9996) and precision (RSD 0.4 and 0.6% ...Continue Reading

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Citations

May 4, 2013·Analytica Chimica Acta·Xiaofang ZhangZhuobin Yuan
Jul 25, 2012·Journal of Chromatography. a·Jonas J P MarkFrank-Michael Matysik
Oct 22, 2011·Journal of Pharmaceutical and Biomedical Analysis·Mohamed N El-AttugAnn Van Schepdael
May 20, 2014·Journal of Separation Science·Sille Stěpánová, Václav Kašička
Jan 8, 2014·Electrophoresis·Elena Domínguez-VegaMaría Luisa Marina
May 8, 2016·Talanta·Oliver Wahl, Ulrike Holzgrabe
Apr 5, 2017·Journal of Chromatographic Science·Bart BlanchaertAnn Van Schepdael

Related Concepts

Electric Conductivity
Kantrex
Electrophoresis, Capillary
Antibiotics, Aminoglycoside
Critical Illness
Histidine
Kanamycin A
Reflex Sympathetic Dystrophy
Silicon Dioxide
Ammonium acetate

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Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.