Capacity of mouse oocytes from preantral follicles to undergo embryogenesis and development to live young after growth, maturation, and fertilization in vitro

Biology of Reproduction
J J Eppig, A C Schroeder

Abstract

A system is described here by which live mice can be produced from oocytes isolated from 12-day-old mice, be grown, matured, and fertilized in vitro, and then be transferred to pseudopregnant females. These oocytes were, at the time of isolation from preantral follicles, in about mid-growth phase and incompetent of undergoing germinal vesicle breakdown (GVB) without further development. The developmental competence of mouse oocytes that grew and underwent maturation in vitro was compared to oocytes that grew in vivo and underwent maturation in vitro. After isolation from mice 16 through 28 days old, oocytes were found to increase in size and to sequentially acquire the ability to undergo GVB, produce a polar body, cleave to the 2-cell stage after insemination, and develop to the blastocyst stage. Moreover, the number of cells per blastocyst increased with the age of the mice from which the immature oocytes were isolated. Oocyte-granulosa cell complexes isolated from 12-day-old mice were cultured for 10 days. At the end of the culture period, the oocytes had grown to a size equivalent to oocytes isolated from 16-day-old mice, and 87% of the in-vitro-grown (IVG) oocytes underwent GVB; 79% of these produced a clearly visible polar...Continue Reading

Citations

Dec 1, 1996·Molecular Reproduction and Development·J SmitzA C Van Steirteghem
May 20, 1998·Molecular Reproduction and Development·B OhB B Knowles
Dec 22, 1999·Molecular Reproduction and Development·J ZhaoR Van Den Hurk
Jul 27, 2000·Molecular Reproduction and Development·K ReynaudM A Driancourt
Nov 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J J Eppig
May 1, 1992·Molecular Reproduction and Development·J J EppigM J O'Brien
Apr 1, 1994·Molecular Reproduction and Development·R D SchrammB D Bavister
Dec 1, 1995·Molecular Reproduction and Development·J J Eppig, K Wigglesworth
Dec 1, 1995·Prenatal Diagnosis·E Pergament, M Fiddler
Jul 1, 1995·Journal of Assisted Reproduction and Genetics·J ZhangM DiMattina
Feb 21, 2013·Journal of Assisted Reproduction and Genetics·Marina C PeluffoMary B Zelinski
Oct 1, 1993·Molecular and Cellular Endocrinology·D Ben-YosefR Shalgi
Oct 3, 2013·Reproductive Biomedicine Online·Roger G Gosden
Dec 25, 2002·Theriogenology·T Miyano
Mar 25, 2000·Theriogenology·E E Telfer
Mar 25, 2000·Theriogenology·X YangG A Presicce
Dec 26, 2001·Archives of Medical Research·P L NayuduS Osborn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.