CAPER 3.0: A Scalable Cloud-Based System for Data-Intensive Analysis of Chromosome-Centric Human Proteome Project Data Sets

Journal of Proteome Research
Shuai YangFuchu He

Abstract

The Chromosome-centric Human Proteome Project (C-HPP) aims to catalog genome-encoded proteins using a chromosome-by-chromosome strategy. As the C-HPP proceeds, the increasing requirement for data-intensive analysis of the MS/MS data poses a challenge to the proteomic community, especially small laboratories lacking computational infrastructure. To address this challenge, we have updated the previous CAPER browser into a higher version, CAPER 3.0, which is a scalable cloud-based system for data-intensive analysis of C-HPP data sets. CAPER 3.0 uses cloud computing technology to facilitate MS/MS-based peptide identification. In particular, it can use both public and private cloud, facilitating the analysis of C-HPP data sets. CAPER 3.0 provides a graphical user interface (GUI) to help users transfer data, configure jobs, track progress, and visualize the results comprehensively. These features enable users without programming expertise to easily conduct data-intensive analysis using CAPER 3.0. Here, we illustrate the usage of CAPER 3.0 with four specific mass spectral data-intensive problems: detecting novel peptides, identifying single amino acid variants (SAVs) derived from known missense mutations, identifying sample-specific S...Continue Reading

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Citations

Dec 17, 2015·Mass Spectrometry Reviews·Gerben Menschaert, David Fenyö
Jul 24, 2015·Journal of Proteome Research·Bharat PanwarYuanfang Guan
Sep 5, 2015·Journal of Proteome Research·Young-Ki PaikWilliam S Hancock
Dec 15, 2015·Molecular & Cellular Proteomics : MCP·Xiaojing WangBing Zhang
Oct 19, 2017·Expert Review of Proteomics·Young-Ki PaikChristopher M Overall

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