PMID: 9451474Feb 6, 1998Paper

Capillary and aortic endothelia interact in situ with nonenzymatically glycated albumin and develop specific alterations in early experimental diabetes

Acta Diabetologica
Doina PopovMaya Simionescu

Abstract

Diabetic mice (6 weeks duration) were studied to assess the interaction of advanced glycation endproduct-modified albumin (AGE-Alb) with micro- and macrovascular endothelium, and to evaluate the alterations induced in the ultrastructure of the lung, kidney, and aorta. [125I]-AGE-Alb and AGE-Alb-Au were perfused in situ in the vasculature; the total uptake was quantitated by spectrometry, and the endothelial pathways of AGE-Alb-Au and the morphological alterations of the vascular beds were examined by electron microscopy. The results showed that [125I]-AGE-Alb (0.567 microM) was taken up specifically and saturably by all organs studied, and particularly by the lung. AGE-Alb-Au endocytosis and transcytosis occurred in the pulmonary and aortic endothelia, and were enhanced in diabetic animals. Also in diabetic animals, AGE-Alb-Au was detected throughout the kidney glomerular basement membrane (GBM) and within open filtration slits of podocytes, suggesting altered barrier function. The structural modifications progressed, and at the end of the experimental period, in the lung approximately 28% of the capillaries and approximately 25% of the alveoli became compressed or even collapsed, due to the hyperplasia of extracellular matrix ...Continue Reading

Citations

Oct 7, 2008·Cell and Tissue Research·Maya SimionescuAnca Sima
Jul 10, 2007·Diabetes Technology & Therapeutics·Connie C W Hsia, Philip Raskin
Jan 12, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·David J FosterConnie C W Hsia
Oct 25, 2007·Platelets·Nicoleta AlexandruManuela Amuzescu
Dec 21, 2006·Archives of Physiology and Biochemistry·Doina Popov, Maya Simionescu
Feb 11, 2011·Diabetes/metabolism Research and Reviews·Junling YangLu Cai
Aug 23, 2005·Biochimica Et Biophysica Acta·Sonia-Athina P KarabinaNalini Santanam
Mar 5, 2005·The American Journal of Medicine·Connie C W Hsia, Philip Raskin
Aug 21, 2010·Diabetic Medicine : a Journal of the British Diabetic Association·O L KleinL J Smith
Apr 14, 2011·American Journal of Physiology. Endocrinology and Metabolism·Junling YangLu Cai
Jul 12, 2002·Microscopy Research and Technique·Maya SimionescuFelicia Antohe
Oct 12, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A W StittH Vlassara
Jul 28, 2020·ERJ Open Research·Emiel F M Wouters, Niki L Reynaert
Dec 18, 2003·Pediatric Pulmonology·Salvatore CazzatoEmanuele Cacciari

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