Capillary-assembled microchip as an on-line deproteinization device for capillary electrophoresis.

Analytical and Bioanalytical Chemistry
Hideaki HisamotoShigeru Terabe

Abstract

A capillary-assembled microchip (CAs-CHIP), prepared by simply embedding square capillaries in a lattice polydimethylsiloxane (PDMS) channel plate with the same channel dimensions as the outer dimensions of the square capillaries, has been used as a diffusion-based pretreatment attachment in capillary electrophoresis (CE). Because the CAs-CHIPs employ square-section channels, diffusion-based separation of small molecules from sample solutions containing proteins is possible by using the multilayer flow formed in the square section channel. When a solution containing high-molecular-weight and low-molecular-weight species makes contact with a buffer solution, the low-molecular-weight species, which have larger diffusion coefficients than the high-molecular-weight species, can be collected in a buffer-solution phase. The collected solution containing the low-molecular-weight species is introduced into the separation capillary to be analyzed by CE. This type of system can be used for CE analysis in which pretreatment is required to remove proteins. In this work a fluorescently labeled protein and rhodamine-based molecules were chosen as model species and a feasibility study was performed.

References

Jun 15, 2004·Analytical Chemistry·Torsten VilknerAndreas Manz
Apr 2, 2005·Analytical Chemistry·Hideaki HisamotoShigeru Terabe
Jun 15, 2005·Analytical Chemistry·Michael G RoperJames P Landers
Aug 29, 2007·Analytica Chimica Acta·Hideaki HisamotoShigeru Terabe
Oct 31, 2008·Talanta·Jan LichtenbergElisabeth Verpoorte

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Citations

Apr 24, 2008·Analytical and Bioanalytical Chemistry·Terence G HenaresHideaki Hisamoto
Jan 12, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Terence G HenaresHideaki Hisamoto
Jan 15, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Terence G HenaresHideaki Hisamoto
Jan 1, 2016·Environmental Chemistry Letters·Imran AliMohd Marsin Sanagi

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Methods Mentioned

BETA
electrophoresis
chip

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