Capillary electrochromatography of biomolecules with on-line electrospray ionization and time-of-flight mass spectrometry

Journal of Chromatography. a
G ChoudharyJ F Banks

Abstract

Capillary electrochromatography (CEC) is considered a hybrid of liquid chromatography and capillary electrophoresis. It is expected to combine the high peak efficiency of capillary zone electrophoresis with the versatility and loading capacity of HPLC to bring about another high-performance MS-compatible chromatographic system. This paper explores the potential of CEC coupled with the electrospray ionization and time-of-flight mass spectrometry in biochemical analysis. The packed columns used in this study were tapered at the outlet to retain the packing material, thereby obviating the need for an outlet frit. Electrosmotically driven solvent gradients were employed for the separation of phenylthiohydantoin (PTH)-amino acids by reversed-phase chromatography, and a time-of-flight (TOF) mass spectrometer was employed as the detector for the CEC column effluent. The effect of CEC operating parameters, such as gradient shape, column length, and electric field, on the analytical results from the separation and MS detection of a standard mixture of PTH-amino acids was investigated. Particular attention was paid to the effect of sheath flow-rate, sheath composition and mass spectra acquisition rate on the performance of the electrospr...Continue Reading

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Citations

Feb 24, 2001·Electrophoresis·L A ColónR L Rodríguez
Feb 28, 2009·Analytical and Bioanalytical Chemistry·Yu-Ping ZhangYi-Xin Zhang
Aug 29, 2000·Journal of Chromatography. a·G ChoudharyW Hancock
Mar 7, 2009·Journal of Separation Science·Bo ZhangPeter Myers
Jan 9, 2007·Rapid Communications in Mass Spectrometry : RCM·Anusak SirikatithamTsutomu Masujima
Feb 20, 2002·Biologicals : Journal of the International Association of Biological Standardization·M T Hearn
May 10, 2016·Journal of Pharmaceutical and Biomedical Analysis·Sven DeclerckDebby Mangelings
Aug 10, 2000·Rapid Communications in Mass Spectrometry : RCM·M GucekJ van der Greef
May 31, 2001·Electrophoresis·A von BrockeE Bayer
Sep 10, 2004·Journal of Chromatography. a·Christian W Klampfl
Oct 20, 1999·Journal of Chromatography. a·K D Altria
Nov 1, 2005·Analytical Chemistry·Dean NortonShahab A Shamsi
Oct 30, 2007·Analytical Chemistry·Bo ZhangPeter Myers
Nov 20, 2001·Chemical Reviews·K B Tomer

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