Capillary electrophoresis based on nucleic acid detection for diagnosing human infectious disease

Clinical Chemistry and Laboratory Medicine : CCLM
Dong-Sheng Lian, Shu-Jin Zhao

Abstract

Rapid transmission, high morbidity, and mortality are the features of human infectious diseases caused by microorganisms, such as bacteria, fungi, and viruses. These diseases may lead within a short period of time to great personal and property losses, especially in regions where sanitation is poor. Thus, rapid diagnoses are vital for the prevention and therapeutic intervention of human infectious diseases. Several conventional methods are often used to diagnose infectious diseases, e.g. methods based on cultures or morphology, or biochemical tests based on metabonomics. Although traditional methods are considered gold standards and are used most frequently, they are laborious, time consuming, and tedious and cannot meet the demand for rapid diagnoses. Disease diagnosis using capillary electrophoresis methods has the advantages of high efficiency, high throughput, and high speed, and coupled with the different nucleic acid detection strategies overcomes the drawbacks of traditional identification methods, precluding many types of false positive and negative results. Therefore, this review focuses on the application of capillary electrophoresis based on nucleic detection to the diagnosis of human infectious diseases, and offers ...Continue Reading

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Citations

Nov 1, 2017·Comprehensive Reviews in Food Science and Food Safety·Dong-Sheng Lian, Hua-Song Zeng

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Methods Mentioned

BETA
electrophoresis
PCR
PCRs
genotyping
lavage
ribotyping
proximity ligation assay
circular dichroism
footprinting
biosensor

Software Mentioned

CE
GeXPS
- FAST
MLVA
MCE
FAST
ICEPlex
SHAPE
Seeplex
ivFAST

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