Capillary electrophoresis combining field-amplified sample stacking and electroosmotic flow suppressant for analysis of sulindac and its two metabolites in plasma

Journal of Chromatography. a
Yen-Ling ChenShou-Mei Wu

Abstract

Field-amplified sample stacking with electroosmotic flow (EOF) suppressant in capillary electrophoresis was used to determine the concentration of sulindac (SU) and its two active metabolites, sulindac sulfide (SI) and sulindac sulfone (SO), in human plasma. After acidification, the analytes were extracted from the plasma with dichloromethane. Before sample loading, a water plug (0.5 psi, 3 s) was injected to contain sample anions and to permit field-amplified stacking. Electrokinetic injection at a reversed voltage (-6 kV, 99.9 s) was then used to introduce anions. Separation was performed using phosphate buffer (80 mM, pH 6.0) containing 2,6-di-O-methyl-beta-cyclodextrin (0.75 mM), and poly(ethylene oxide) (0.01%) as an EOF suppressant. The separation was performed at -30 kV and 200 nm. During method validation, calibration plots were linear (r > 0.994) over a range of 0.3-30.0 microM for SU and SO, and 0.5-30.0 microM for SI. During intra- and inter-day analysis, relative standard deviations (RSD) and relative errors (RE) were all less than 16%. The limits of detection were 0.1 microM for SU and SO, and 0.3 microM for SI (S/N = 4, sampling 99.9s at -6 kV). This method was feasible for determining SU and its metabolites in pl...Continue Reading

References

Feb 5, 2002·Electrophoresis·Bojeong Kim, Doo Soo Chung
Nov 10, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Samir E WittaS Gail Eckhardt

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Citations

Nov 24, 2007·Journal of Chromatography. a·Steven L SimpsonShigeru Terabe
Dec 23, 2008·Electrophoresis·Zdena MaláPetr Bocek
Sep 29, 2006·The Journal of Pharmacology and Experimental Therapeutics·Azza A K El-SheikhFrans G M Russel
Jun 15, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yu-Ling KuoHsi-Ya Huang

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