Capillary electrophoresis coupled with electrochemiluminescence detection using porous etched joint

Analytical Chemistry
Xue-Bo YinErkang Wang

Abstract

A new setup to couple capillary electrophoresis (CE) with electrochemiluminescence (ECL) detection is described in which the electrical connection of CE is achieved through a porous section at a distance of 7 mm from the CE capillary outlet. Because the porous capillary wall allowed the CE current to pass through and there was no electric field gradient beyond that section, the influence of CE high-voltage field on the ECL procedure was eliminated. The porous section formed by etching the capillary with hydrofluoric acid after only one side of the circumference of 2-3 mm of polyimide coating of the capillary was removed, while keeping the polyimide coating on the other part to protect the capillary from HF etching makes the capillary joint much more robust since only a part of the circumference of it is etched. A standard three-electrode configuration was used in experiments with Pt wire as a counter electrode, Ag/AgCl as a reference electrode, and a 300-microm diameter Pt disk as a working electrode. Compared with CE-ECL conventional decoupler designs, the present setup with a porous joint has no added dead volume created. Moreover, the dead volume can be increasingly decreased by shortening the distance ( approximately 100 mi...Continue Reading

Citations

Dec 25, 2010·Analytical and Bioanalytical Chemistry·Longhua GuoGuonan Chen
Dec 16, 2011·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Jing Li, Erkang Wang
Oct 21, 2009·Journal of Separation Science·Zhifeng FuWei Wei
Jul 2, 2010·Chemical Society Reviews·Lianzhe Hu, Guobao Xu
Jun 22, 2010·Electrophoresis·Francisco J LaraAurelia Ibáñez Velasco
Jul 12, 2013·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Qing-Wei ZhouJia-Jun Chen
Jan 1, 2011·Analytical Methods : Advancing Methods and Applications·Guo GuangmeiXi Chen

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