Capillary electrophoresis investigation of a partially unfolded conformation of beta(2)-microglobulin

Electrophoresis
Ersilia De LorenziGabriele Caccialanza

Abstract

Dialysis-related amyloidosis is a disease in which partial unfolding of beta(2)-microglobulin plays a key pathogenetic role in the formation of the amyloid fibrils. We have recently demonstrated that a partially unfolded conformer of beta(2)-microglobulin is involved in fibrillogenesis and that this species is significantly populated under physiological conditions. In this work capillary electrophoresis has been used to measure the equilibrium between the native protein and this conformer in samples known to have a higher or lower amyloidogenic potential, namely full-length beta(2)-microglobulin, two truncated species and a mutant, created by replacing histidine in position 31 with thyrosine. In addition, for all protein species folding stability experiments have been carried out by monitoring the secondary structure by circular dichroism at increasing concentrations of guanidinium chloride. The values of free energy of unfolding in the absence of denaturant, obtained by elaboration of these experiments, were found to be inversely correlated to the area percent of the partially unfolded conformer, as measured by capillary electrophoresis. Affinity capillary electrophoresis experiments have been also carried out under nondenatur...Continue Reading

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Citations

Jun 5, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ondrea Bermudez, Daniel Forciniti
Dec 19, 2002·Journal of Molecular Biology·Susan JonesSheena E Radford
Jul 10, 2003·Journal of Molecular Biology·Neil M KadNeil H Thomson
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Aug 7, 2009·Amyloid : the International Journal of Experimental and Clinical Investigation : the Official Journal of the International Society of Amyloidosis·Niels H H Heegaard
Jun 12, 2009·Electrophoresis·Niels H H Heegaard
Jun 1, 2007·Electrophoresis·Maria V PiaggioJulio A Deiber
Mar 31, 2012·Therapeutic Apheresis and Dialysis : Official Peer-reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy·Yoshinori UjiYukio Ando
Jul 24, 2012·Therapeutic Apheresis and Dialysis : Official Peer-reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy·Yoshihiro MotomiyaYukio Ando
Nov 25, 2004·Journal of Chromatography. a·Hui XuHong-Yuan Chen
Aug 23, 2005·Biochimica Et Biophysica Acta·Niels H H Heegaard, Ersilia De Lorenzi
Aug 9, 2005·Biochimica Et Biophysica Acta·Camillo RosanoMartino Bolognesi
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Nov 17, 2012·Journal of Molecular Biology·Atsushi MukaiyamaKunihiro Kuwajima
Dec 9, 2003·The Journal of Biological Chemistry·Alessandra CorazzaGennaro Esposito

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