Capillary electrophoresis-mass spectrometry of a new cross-linker with acrylic functionality

Electrophoresis
Carolina SimóAlejandro Cifuentes

Abstract

Analytical characterization of dimethacrylate-tyrosine-lysine-tyrosine (DMTLT, a new biodegradable acrylic cross-linker synthesized at our laboratory) is carried out using CE-MS. DMTLT is a pseudopeptide composed by tyrosine-lysine-tyrosine amino acids linked through urea bonds with two methacrylic groups, one at each end of the molecule, making this compound an excellent cross-linker for polymerization reactions and for obtaining new biodegradable materials. A new CE-MS method is developed for the characterization of DMTLT and its products of degradation after basic hydrolysis. In order to carry out an exhaustive examination of such degradation products methods based on CE coupled to IT and TOF-MS are employed. Based on CE-IT-MS results and the elemental composition of the degradation products obtained by CE-TOF-MS, conclusions on the mechanism and kinetic of hydrolysis of DMTLT are obtained confirming both the usefulness of CE-MS to characterize new biomaterials and the applicability of DMTLT for preparing new biodegradable polymers. These results are corroborated through the CE-MS detection of the identified products of degradation in a dimethyl acrylamide polymer cross-linked with DMTLT.

References

Jan 1, 1990·Journal of Biomaterials Science. Polymer Edition·V SubrJ Kopecek
Jan 1, 1994·Journal of Biomaterials Science. Polymer Edition·R S LabowJ P Santerre
Sep 30, 1994·Journal of Chromatography. a·A Cifuentes, H Poppe
Mar 14, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·A GallardoJ San Román
Feb 28, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·C SimóA Cifuentes
Dec 3, 2002·Tissue Engineering·Jian-Ying ZhangJeffrey O Hollinger
Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Carolina SimóAlberto Gallardo
Dec 9, 2003·Electrophoresis·Philippe Schmitt-Kopplin, Moritz Frommberger
Jan 15, 2004·Analytical Chemistry·Carolina SimóAlejandro Cifuentes
Jun 24, 2004·Journal of Biomaterials Science. Polymer Edition·Christian Braud
Jul 27, 2004·Electrophoresis·Javier Hernández-BorgesMatthias Pelzing
Mar 12, 2005·Electrophoresis·Carolina SimóAlejandro Cifuentes
Apr 23, 2005·Journal of Chromatography. a·Hervé CottetAlejandro Cifuentes
Oct 1, 1999·Annals of the New York Academy of Sciences·Agnes JaniMichael E Shy

❮ Previous
Next ❯

Citations

Jun 7, 2008·Journal of Biomaterials Science. Polymer Edition·Paloma PérezJulio San Román
May 15, 2009·Electrophoresis·Aline StaubJean-Luc Veuthey
Jun 12, 2009·Electrophoresis·Christian W Klampfl
Jan 22, 2009·Electrophoresis·Pavla PantůckováLudmila Krivánková
Apr 15, 2008·Electrophoresis·Miguel HerreroAlejandro Cifuentes
Feb 28, 2007·Journal of Mass Spectrometry : JMS
Dec 29, 2007·Electrophoresis·Andras GasparPhilippe Schmitt-Kopplin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.