Capillary electrophoresis-mass spectrometry of basic proteins using a new physically adsorbed polymer coating. Some applications in food analysis

Electrophoresis
Carolina SimóAlejandro Cifuentes

Abstract

A new physically adsorbed capillary coating for capillary electrophoresis-mass spectrometry (CE-MS) of basic proteins is presented, which is easily obtained by flushing the capillary with a polymer aqueous solution for two min. This coating significantly reduces the electrostatic adsorption of a group of basic proteins (i.e., cytochrome c, lysozyme, and ribonuclease A) onto the capillary wall allowing their analysis by CE-MS. The coating protocol is compatible with electrospray inonization (ESI)-MS via the reproducible separation of the standard basic proteins (%RSD values (n = 5) < 1% for analysis time reproducibility and < 5% for peak heights, measured from the total ion electropherograms (TIEs) within the same day). The LODs determined using cytochrome c with total ion current and extracted ion current defection were 24.5 and 2.9 fmol, respectively. Using this new coating lysozymes from chicken and turkey egg white could be easily distinguished by CE-MS, demonstrating the usefulness of this method to differentiate animal species. Even after sterilization at 120 degrees C for 30 min, lysozyme could be detected, as well as in wines at concentrations much lower than the limit marked by the EC Commission Regulation. Adulteration...Continue Reading

Citations

Aug 16, 2008·Analytical and Bioanalytical Chemistry·Jochen MetzgerHarald Mischak
Oct 20, 2009·Analytical and Bioanalytical Chemistry·C HuhnG W Somsen
Aug 18, 2010·The Analyst·Makedonka D GulcevCharles A Lucy
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Jan 27, 2005·Journal of Mass Spectrometry : JMS
Mar 11, 2005·Electrophoresis·Carolina SimóAlejandro Cifuentes
Mar 12, 2005·Electrophoresis·Carolina SimóAlejandro Cifuentes
Aug 12, 2008·Journal of Chromatography. a·José BernalAlejandro Cifuentes
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Oct 27, 2007·Electrophoresis·Guillaume L ErnyAlejandro Cifuentes
Jan 22, 2009·Electrophoresis·Pavla PantůckováLudmila Krivánková
Apr 29, 2006·Electrophoresis·Carolina SimóAlejandro Cifuentes
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