Capillary electrophoresis method optimized with a factorial design for the determination of glutathione and amino acid status using human capillary blood

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Gordana Zunić, Slavica Spasić

Abstract

Plasma aromatic and sulfur containing amino acids are good indicators of protein anabolism/catabolism, while blood reduced and oxidized glutathione reflect oxidative status in an organism. Using a full factorial design for screening important variables (pH, concentration, temperature) we developed a capillary zone electrophoresis method permitting their measurements in the single run, without any derivatization procedures. The best separations were obtained within less than 30 min employing a 10 mmol/l phosphate buffer, pH 2.8, 18 degrees C, 15 kV voltage. Fairly good precision with a linear relationship between peak area and concentrations (r=0.995-0.999) were obtained. The method was used to analyze human capillary blood.

References

Dec 5, 1998·World Journal of Surgery·I CernakV Stepic
May 23, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Gordana ZunićSlavica Spasić
Dec 4, 2003·Free Radical Biology & Medicine·Daniela GiustariniRanieri Rossi
Jun 28, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·N MaesoC Barbas
Jun 1, 2007·Electrophoresis·Susanne Kopec, Ulrike Holzgrabe

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Citations

Aug 5, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Filippo Carlucci, Antonella Tabucchi
Oct 27, 2014·Journal of Chromatographic Science·Roman KanďárMartina Brandtnerová
May 15, 2015·Journal of Agricultural and Food Chemistry·Elena MarcoliniFrancesco Capozzi
Oct 3, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zaneta BuchtovaOndrej Zitka
Dec 8, 2009·Electrophoresis·Véréna PoinsotFrançois Couderc
Dec 2, 2009·Electrophoresis·Václav Kasicka
Sep 10, 2016·Electrophoresis·Júlia LačnáPetr Kubáň
Jul 8, 2011·Analytical Chemistry·Geoffrey P McDermottPaul S Francis
Jun 30, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Péter MonostoriSándor Túri

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