Capillary electrophoresis microchips for separation and detection of organophosphate nerve agents

Analytical Chemistry
J WangW Chen

Abstract

A miniaturized analytical system for separating and detecting toxic organophosphate nerve agent compounds, based on the coupling of a micromachined capillary electrophoresis chip with a thick-film amperometric detector, is described. Factors influencing the on-chip separation and detection processes have been optimized. Using a MES buffer (20 mM, pH 5.0) running buffer, a 72-mm-long separation channel, and a separation voltage of 2000 V, baseline resolution is observed for paraoxon, methyl parathion, fenitrothion, and ethyl parathion in 140 s. Such miniaturization and speed advantages are coupled to submicromolar detection limits and good precision. Applicability to spiked river water samples is demonstrated, and the implications for on-site environmental monitoring and rapid security screening/warning are discussed.

References

Mar 10, 1998·Analytical Chemistry·A T WoolleyR A Mathies
May 18, 2000·Analytical Chemistry·D Figeys, D Pinto
Oct 12, 2000·Analytical Chemistry·S A SoperM C Murphy

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Citations

Aug 25, 2001·Electrophoresis·N A LacherS M Lunte
Mar 3, 2005·Analytical and Bioanalytical Chemistry·Chunya LiShengshui Hu
Oct 6, 2001·Journal of Chromatography. a·M A Schwarz, P C Hauser
Sep 24, 2002·Biosensors & Bioelectronics·Miguel RodriguezElias Greenbaum
Nov 20, 2008·Journal of Exposure Science & Environmental Epidemiology·Richard C BarryCharles A Timchalk
May 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Malin AllertHomme W Hellinga
Feb 7, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Yingning GaoIan Wheeldon
Jan 23, 2015·Nanoscale·Muhammad Zafir Mohamad NasirMartin Pumera
Aug 23, 2006·Journal of Separation Science·Barbora PapouskováKarel Lemr
Feb 23, 2008·Journal of Separation Science·Fumihiko Kitagawa, Koji Otsuka
May 24, 2008·Lab on a Chip·Hsih Yin TanNam-Trung Nguyen
Oct 16, 2015·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Flavio PinoYasuaki Einaga
Jan 1, 2016·Environmental Chemistry Letters·Imran AliMohd Marsin Sanagi
Jun 13, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·LiPing HuPinGang He
Aug 23, 2011·The Analyst·Svetlana S AleksenkoAndrei R Timerbaev
Feb 18, 2011·Advanced Materials·Mustafa MusamehStephen C Hawkins
Mar 3, 2021·Analytica Chimica Acta·Patricia Ramirez-PriegoLaura M Lechuga

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