PMID: 9527853May 2, 1998Paper

Capillary electrophoresis of insulin-like growth factors: enhanced ultraviolet detection using dynamically coated capillaries and on-line solid-phase extraction

Analytical Biochemistry
M E RocheJ P Landers

Abstract

Insulin-like growth factor-I and -II (IGF-I and IGF-II) are difficult to separate and measure as a result of their homology, both structurally and immunologically. A number of binding proteins (BPs) which interact with the IGFs with high affinity complicate the ability to measure the IGFs accurately and reproducibly. Current methodology for measuring IGF is immuno-based and involves dissociation from the IGFs and removal of the binding proteins through sample acidification and removal by solid-phase adsorption. However, the net result is an assay that is time-consuming and, at best, semiquantitative. In an attempt to improve the reproducibility and accuracy of IGF-I and -II measurement, electrophoretic systems employing dynamically coated and bare silica capillaries were evaluated. Separations in bare silica capillaries in the presence or absence of the cationic additive, decamethonium bromide were ineffective for resolving IGF-I and IGF-II. However, when the capillary was coated dynamically with polybrene, IGF-I and -II could be resolved in a BSA sample matrix using a low pH buffer. Despite the fact that the IGFs could be resolved in the presence of an IGF-I analog used as an internal standard, polybrene recoating was required...Continue Reading

References

Sep 1, 1985·Calcified Tissue International·P G Robey, J D Termine
Oct 21, 1983·Science·J W Jorgenson, K D Lukacs

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Citations

Dec 7, 2007·Electrophoresis·F W Alexander TempelsGerhardus J de Jong
Apr 12, 2001·Electrophoresis·J Horvath, V Dolník
Dec 22, 1999·Electrophoresis·V Kasicka
Feb 20, 2007·Electrophoresis·Grégoire DangerHervé Cottet
Aug 28, 2001·Journal of Chromatography. a·M ValcárcelA Ríos
Jul 6, 2000·Analytical Chemistry·S N Krylov, N J Dovichi

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