Capillary electrophoresis of peptides and proteins in fused-silica capillaries coated with derivatized polystyrene nanoparticles

Electrophoresis
G KleindienstG K Bonn

Abstract

High-resolution capillary electrophoretic separation of proteins and peptides was achieved by coating the inner wall of 75 microm ID fused-silica capillaries with 40-140 nm polystyrene particles which have been derivatized with alpha-omega-diamines such as ethylenediamine or 1,10-diaminodecane. A stable and irreversibly adsorbed coating was obtained upon deprotonation of the capillary surface with aqueous sodium hydroxide and subsequent flushing with a suspension of the positively charged particles. At pH 3.1, the detrimental adsorption of proteins to the capillary inner wall was suppressed efficiently because of electrostatic repulsion of the positively charged proteins from the positively charged coating which enabled protein separations with maximum efficiencies of 400000 plates per meter. A substantial improvement of separation efficiency in particle-coated capillaries was observed after in-column derivatization of amino functionalities with 2,3-epoxy-l-propanol, resulting in a more hydrophilic coating. Five basic and four acidic proteins could be separated in less than 7 min with efficiencies up to 1900000 theoretical plates per meter. Finally, coated capillaries were applied to the high-resolution analysis of protein glyc...Continue Reading

References

Apr 21, 1976·Journal of Chromatography·P G Righetti, T Caravaggio
Jun 1, 1991·Analytical Chemistry·J K Towns, F E Regnier
Sep 7, 1990·Journal of Chromatography·J K Towns, F E Regnier
Sep 1, 1990·Electrophoresis·J E Wiktorowicz, J C Colburn
Oct 21, 1983·Science·J W Jorgenson, K D Lukacs
Feb 3, 1995·Journal of Chromatography. B, Biomedical Applications·M LeonardE Dellacherie
May 1, 1993·Analytical Biochemistry·M A Strege, A L Lagu

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Citations

Oct 20, 2009·Analytical and Bioanalytical Chemistry·C HuhnG W Somsen
Oct 25, 2000·Journal of Chromatography. a·M C BreadmoreP R Haddad
Jul 13, 2000·Journal of Chromatography. a·B G BelenkiiD G Nasledov
Apr 12, 2007·Expert Review of Proteomics·Yanqing WangJoris R Delanghe
Nov 27, 2004·Electrophoresis·Vladislav Dolník
Aug 28, 2007·Journal of Chromatography. a·Christian NilssonStaffan Nilsson
Jun 9, 2005·Journal of Separation Science·Li YangJeremy D Glennon
Feb 18, 2010·Electrophoresis·Stefan BachmannGünther K Bonn
Apr 12, 2001·Electrophoresis·J Horvath, V Dolník
Jul 14, 2017·Electrophoresis·Vojtech Adam, Marketa Vaculovicova
Sep 18, 2001·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Q ShiK Xie
Aug 4, 2006·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·N JanduM W C Hatton
Jan 31, 2002·Analytical Chemistry·M PumeraR Polsky
Jul 6, 2000·Analytical Chemistry·S N Krylov, N J Dovichi

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