Capillary electrophoresis tandem mass spectrometry determination of glutamic acid and homocysteine's metabolites: Potential biomarkers of amyotrophic lateral sclerosis

Talanta
Zuzana CieslarovaAna Valéria Colnaghi Simionato

Abstract

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease that affects both lower and upper motor neurons, leading to muscle atrophy, paralysis, and death caused by respiratory failure or infectious complications. Altered levels of homocysteine, cysteine, methionine, and glutamic acid have been observed in plasma of ALS patients. In this context, a method for determination of these potential biomarkers in plasma by capillary electrophoresis tandem mass spectrometry (CE-MS/MS) is proposed herein. Sample preparation was carefully investigated, since sulfur-containing amino acids may interact with plasma proteins. Owing to the non-thiol sulfur atom in methionine, it was necessary to split sample preparation into two methods: i) determination of homocysteine and cysteine as S-acetyl amino acids; ii) determination of glutamic acid and methionine. All amino acids were separated within 25min by CE-MS/MS using 5molL-1 acetic acid as background electrolyte and 5mmolL-1 acetic acid in 50% methanol/H2O (v/v) as sheath liquid. The proposed CE-MS/MS method was validated, presenting RSD values below 6% and 11% for intra- and inter-day precision, respectively, for the middle concentration level within the linear range. The limits of ...Continue Reading

Citations

Sep 21, 2018·Electrophoresis·Rawi RamautarGerhardus J de Jong
Jun 17, 2020·Expert Review of Neurotherapeutics·Roberta BonomoDebra J Skene
Aug 15, 2017·Electrophoresis·Véréna PoinsotFrançois Couderc
Jan 9, 2019·Frontiers in Neurology·Débora LanznasterHélène Blasco
Apr 10, 2019·Critical Reviews in Analytical Chemistry·Raquel Pérez-MíguezMaría Luisa Marina
Sep 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Juraj PiestanskyPeter Mikus
Feb 23, 2020·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Yonghua WuTiancheng Wang
May 30, 2019·Frontiers in Neurology·Udaya Geetha VijayakumarStephanie Marie-Rose Duguez
Jul 13, 2021·Analytica Chimica Acta·Hai Yen TaFrancois Couderc

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