Capillary electrophoresis-tandem mass spectrometry for multiclass analysis of polar marine toxins

Analytical and Bioanalytical Chemistry
Daniel G BeachPearse McCarron

Abstract

Polar marine toxins are more challenging to analyze by mass spectrometry-based methods than lipophilic marine toxins, which are now routinely measured in shellfish by multiclass reversed-phase liquid chromatography-tandem mass spectrometry (MS/MS) methods. Capillary electrophoresis (CE)-MS/MS is a technique that is well suited for the analysis of polar marine toxins, and has the potential of providing very high resolution separation. Here, we present a CE-MS/MS method developed, with use of a custom-built interface, for the sensitive multiclass analysis of paralytic shellfish toxins, tetrodotoxins, and domoic acid in seafood. A novel, highly acidic background electrolyte (5 M formic acid) was designed to maximize protonation of analytes and to allow a high degree of sample stacking to improve the limits of detection. The method was applied to a wide range of regulated and less common toxin analogues, and exhibited a high degree of selectivity between toxin isomers and matrix interference. The limits of detection in mussel tissue were 0.0052 mg/kg for tetrodotoxins, 0.160 mg/kg for domoic acid, and between 0.0018 and 0.120 mg/kg for paralytic shellfish toxins, all of which showed good linearity. Minimal ionization suppression wa...Continue Reading

References

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Oct 6, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jeremy Sobel, John Painter
Aug 18, 2010·Marine Drugs·Maria WieseBrett A Neilan
May 6, 2011·Aquatic Toxicology·Rocky ChauBrett A Neilan
Feb 26, 2014·Toxins·Vaishali BaneAmbrose Furey
Aug 19, 2016·Rapid Communications in Mass Spectrometry : RCM·Daniel G BeachPearse McCarron

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