PMID: 9188836May 9, 1997Paper

Capillary electrophoresis with laser-induced fluorescence detection, an adequate alternative to high-performance liquid chromatography, for the determination of ciprofloxacin and its metabolite desethyleneciprofloxacin in human plasma

Journal of Chromatography. B, Biomedical Sciences and Applications
K H BannefeldG Blaschke

Abstract

A method to determine plasma concentrations of ciprofloxacin and its metabolite desethyleneciprofloxacin (M1) by CE with HeCd laser-induced fluorescence detection is described. Following precipitation of proteins and centrifugation supernatant is injected hydrodynamically (10 s, 0.5 p.s.i.) into the capillary. Overall analysis time for the quantification of both analytes was 7 min. The total amount of plasma needed for multiple injections (n>5) was 10-20 microl. Data on accuracy and precision are presented. The assay performance is compared to the specifications of a validated HPLC method, which is routinely used for the quantification of ciprofloxacin and M1 in body fluids. Both methods showed comparable accuracy and precision for both analytes throughout the whole working range (inter-day precision <9%; inter-day accuracy 96-110%). The limit of quantification (LOQ) of 20 microg/l (M1 10 microg/l) for the CE procedure was slightly higher than for the HPLC method, where 10 microg/l (M1 2.5 microg/l) was determined. However, application of the methods to human plasma samples derived from a clinical study proved that comparable results are obtained and that the sensitivity of the HPCE method was sufficient to fully describe typic...Continue Reading

References

Jan 1, 1994·Physical Review C: Nuclear Physics·P HuiJ A Faucett

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Citations

Dec 22, 1999·Electrophoresis·S H Chen, Y H Chen
Dec 22, 1999·Electrophoresis·C L Flurer
Jan 22, 2010·Analytical and Bioanalytical Chemistry·Manuel Lombardo-AgüíCarmen Cruces-Blanco
Jun 16, 2005·International Journal of Antimicrobial Agents·Andries GousHeino Stass
Jun 7, 2002·Journal of Pharmaceutical and Biomedical Analysis·S FurlanettoS Pinzauti
Mar 12, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·D BarrónJ Barbosa
May 23, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Margarita HernándezMarta Calull
Dec 1, 2000·The Pediatric Infectious Disease Journal·E LeibovitzR Dagan
Nov 19, 2002·Antimicrobial Agents and Chemotherapy·Martin BrunnerMarkus Müller
May 25, 2004·Antimicrobial Agents and Chemotherapy·Alison K MeagherJerome J Schentag
Dec 2, 2011·Chemical & Pharmaceutical Bulletin·Marianne Alphonse Mahrouse, Ehab Farouk Elkady
Feb 7, 2016·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Khalid A M AttiaAhmed Serag
Mar 6, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Han-Wen SunShu-Xuan Liang
Mar 29, 2005·Journal of Separation Science·Victoria F SamanidouIoannis N Papadoyannis
May 24, 2006·Electrophoresis·Rukhsana JabeenJohn Petersen
Nov 10, 2004·Journal of Chromatography. a·Katarzyna MichalskaStefan Tyski
Sep 8, 2004·Journal of Pharmaceutical and Biomedical Analysis·Hongyuan YanYi Chen
Jun 21, 2006·Electrophoresis·Ulrike HolzgrabeYaser Bitar

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