Capillary electrophoretic determination of the constituents of paeoniae radix

Journal of Chromatography. a
H K Wu, S J Sheu

Abstract

A method combining the techniques of capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) has been developed to separate a total of eight peony constituents. The CZE method was used to determine the content of paeonol, oxypaeoniflorin, benzoic acid, pentagalloylglucose and gallic acid, and MEKC technique based on sodium cholate was applied to analyze albiflorin, paeoniflorin, benzoyla biflorin, paeonol and oxypaeoniflorin. Linearity around two orders of magnitude of concentration was generally obtained and limits of detection for these compounds were in the range of 2.6-23.7 micrograms/ml. The relative standard deviations of migration times were less than 1.43% (n = 6). Contents of peony constituents in an ethanol-water extract of Paeonia lactiflora Pall. sample could easily be determined by this method.

References

Aug 1, 1982·Planta medica·M Takechi, Y Tanaka

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Citations

Jun 26, 2003·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yukari KuwaharaHiroyuki Nishi
Mar 26, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Gang ChenPengyuan Yang
Oct 7, 2015·Critical Reviews in Analytical Chemistry·Felipe Hugo Alencar Fernandes, Hérida Regina Nunes Salgado
Nov 3, 2006·Biomedical Chromatography : BMC·Ben LiuBo Shen
Jun 22, 2018·ChemMedChem·Srinivasa Reddy BonamVijayapandi Pandy
Dec 1, 2006·Electrophoresis·Peng LiYi Tao Wang
Aug 24, 2005·Archives of Pharmacal Research·Seung Chul LeeMoon Young Heo
Mar 1, 2011·Analytical Methods : Advancing Methods and Applications·Hongyi Zhang, Hui Chen

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